3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1989, october 30th to 1989, november 2nd

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study performed according to the Test Guidelines described in the EEC Directive 84/449/EEC.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1990

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide

EC Number:

406-200-8

EC Name:

3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide

Cas Number:

117827-06-2

Molecular formula:

C24H39Cl2NO2

IUPAC Name:

N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)hexadecanamide

Details on test material:

Identification : UC—136
RCC NOTOX Substance : 1763
RCC NOTOX old project : 1343
Description : White powder
Purity : 98.5%
Instructions for test article storage : At room temperature in the dark in a tightly sealed container
Stability of test article : Stable for one year maximal under storage conditions
Expiry date : January 5, 1990
Stability in vehicles : Unknown
Safety Precautions : Gloves, goggles and face mask were sufficient to assure personnel health and safety.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Species : Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
Breeding : Synchronously in DSW with a density of 25 daphnia per litre in a climate room.
Start of’ each batch : With newborn animals, i.e. less than 3 days old, by placing about 250 of them into 10
litres of DSW in an all—glass culture vessel.
Maximum age of the cultures : 4 weeks
Renewal of the cultures : After 7 days of cultivation half of the volume medium twice a week.
Reason for selection : This system has been selected as an internationally accepted species and
recommended by the referred guidelines.
Medium : Dutch standard water (DSW): formulated using Milli—Q water according to Adema (Adema
D.M.M., 1980; in: Degradability, Eco—toxicity and Bioaccumulation: The determination of the
possible effects of chemicals and wastes on the aquatic environment, Chapter 5,
Government Publishing Office, The Hague, The Netherlands).
Milli—Q water: tap water purified by reverse osmosis and subsequently passed over
activated carbon and ion—exchange cartridges (Millipore Corp., Bedford, Mass., USA).
Temperature : 20± 2°C
Feed : A suspension of fresh water algae.
Frequency : Once a day
Characteristics : For the test selection of young daphnia with an age of <24 hours.
Validity of batch : Frequent inspection of the cultures with respect to number of young, appearance of
young and parental daphnia and global feeding behaviour.
Control of sensitivity : In a 24—hour reference test with potassium di chromate.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Test conditions

Hardness:

11.7°DH

Test temperature:

19.0 to 20.0 °C

pH:

7.8 to 8.3

Dissolved oxygen:

> 5 mg/L

Salinity:

Ca 2+ 54.5 mg/L
Mg 2+ 17.8 mg/L
Na + 27.4 mg/L
K + 7.8 mg/L
Cl - 96.3 mg/L
a) C03 2- ---
a) HC03 - 84.8 mg/L
S04 2- 70.2 mg/L

Nominal and measured concentrations:

an oversaturated solution of 1000 mg/L unfiltrated and filtrated (=saturated solution)

Details on test conditions:

Identification : The vessels were individually identified by means of adhesive labels as described in
detail in RCC NOTOX’s Standard Operating Procedures.
Method : As described in the guideline with some modifications, in accordance with the standard operation procedure: SOP ET/1641.
Test type : Limit test, static
Test duration : 24 hours
Test vessels : 250 ml, all—glass
Test medium : Dutch standard water.
Number of daphnia : 20 per concentration
Loading : 10 per vessel
Light : 16 hours photoperiod daily
Room temperature : 20.0°C
Feeding : No feeding
Concentration range : Because the test substance was hardly soluble in water a limit study was performed, i.e.
exposure to an oversaturated solution of 1000 mg/l unfiltrated and filtrated (=saturated solution).
Controls : Blank:test medium without test substance or other additives.
Treatment stock solutions : The test substance was hardly soluble in water. Preparation of the stock solution:
958.4 mg of test substance was quantitatively added to 1000 ml medium providing a stock
solution of 1000 mg/l. This oversaturated stock solution was stirred for 48 hours.
Subsequently, from this solution 200 ml was taken and filtrated over a 45 um filter and
as such tested, Le. 2x100 ml divided over 2 vessels. From the stirred solution another 2x100 ml
were taken and tested without filtration. In these solutions precipitation of test substance was present.
Introduction of young daphnia : The daphnia were introduced into the test
medium within approximately 30 minutes after preparation of the test media.

Reference substance (positive control):

yes

Remarks:

The reference substance potassium dichromate was tested at the same time as the final test with UC—136, using daphnia from the same culture. The 24—hour EC5O was 1.04 mg/l, which is within the expected range: 0.56—1.91 mg/l (n=20).

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study the test substance UC—136
appears to induce no immobility when Daphnia magna are exposed to either a
saturated or an oversaturated solution.