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EC number: 201-398-0 | CAS number: 82-16-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- A dose of 15000 mg/kg bw of the test substance was applied once to 10 female Wistar rats per gavage. The animals were inspected several times on the day of administration, and twice daily during the following 14-day observation
period (once on Weekends and bank holidays). During inspections, the type, onset, duration, and intensity of clinical signs were recorded and dead animals removed if necessary. The animals were individually weighed at application at the end of the 14-day observation period. - GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 1,8-bis[(4-methylphenyl)amino]anthraquinone
- EC Number:
- 201-398-0
- EC Name:
- 1,8-bis[(4-methylphenyl)amino]anthraquinone
- Cas Number:
- 82-16-6
- Molecular formula:
- C28H22N2O2
- IUPAC Name:
- 1,8-bis[(4-methylphenyl)amino]-9,10-dihydroanthracene-9,10-dione
Constituent 1
- Specific details on test material used for the study:
- Purity: technically pure
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- The acute toxicity experiment was carried out with SPF-bred Wistar rats (strain Wistar TNO W 74). At the start of study the female rats were about 14 weeks old and females 14weeks.
The rats were housed in groups of five animals each under conventional conditions in Makrolon Type-III cages on dust-free wood granules; they were exposed to a room temperature of 22 ± 1.5° C, a 12-hour light/dark cycle (artificial light from 7 a.m. to 7 p.m. CET), and relative humidity of about 60 ± 5 %.
During the study period the animals received feed and tap water ad libitum.
The animals were inspected several times on the day of administration, and twice daily during the following 14-day observation period (once on Weekends and bank holidays). During inspections, the type, onset, duration, and intensity of clinical signs were recorded and dead animals removed if necessary. The animals were individually weighed at application and at the end of the 14-day observation period.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- peanut oil
- Details on oral exposure:
- The substance was formulated inpeanut oil and once administered to 10 female animals at a constant application volume of 30 ml/kg body weight. A rigid metal gavage was used for that purpose. The animals were inspected several times on the day of administration, and twice daily during the following 14-day observation period (once on Weekends and bank holidays). During inspections, the type, onset, duration, and intensity of clinical signs were recorded and dead animals removed if necessary. The animals were individually weighed at application and at the end of the 14-day observation period.
- Doses:
- 15000 mg/kg bw
- No. of animals per sex per dose:
- 10 female rats/dose
- Control animals:
- no
- Details on study design:
- A dose of 15000 mg/kg bw of the test substance was applied once to 10 female Wistar rats per gavage. The animals were inspected several times on the day of administration, and twice daily during the following 14-day observation
period (once on Weekends and bank holidays). During inspections, the type, onset, duration, and intensity of clinical signs were recorded and dead animals removed if necessary. The animals were individually weighed at application at the end of the 14-day observation period.
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- discriminating dose
- Effect level:
- 15 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- None of the animals died.
- Clinical signs:
- other: other: The dose of 15000 mg/kg body eight, which was administered once, was tolerated without symptoms.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LD50 is greater than 15000 mg/kg bw (discriminating dose).
- Executive summary:
A dose of 15000 mg/kg bw of the test substance was applied once to 10 female Wistar rats per gavage. The animals were inspected several times on the day of administration, and twice daily during the following 14-day observation period. The dose of 15000 mg/kg body weight, which was administered once, was tolerated without symptoms by all female animals. The LD50 is greater than 15000 mg/kg bw (discriminating dose).
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