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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is fom peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Dominant lethal mutagenicity study on hair dyes
Author:
C. Burnett, R. Loehr & J. Corbett
Year:
1977
Bibliographic source:
Journal of Toxicology and Environmental Health, 2:3, 657-66, 1977

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as below
Principles of method if other than guideline:
The test material was tested for percutaneous toxicity in groups of four to eight New Zealand rabbits (1.7-2.5 kg). The rabbits were shaved closely the day before the materials were applied in 10 ml of an oxidation-type hair dye base. The period of application was 24 hr.
GLP compliance:
not specified
Test type:
other: no data

Test material

Constituent 1
Chemical structure
Reference substance name:
2-nitro-p-phenylenediamine
EC Number:
226-164-5
EC Name:
2-nitro-p-phenylenediamine
Cas Number:
5307-14-2
Molecular formula:
C6H7N3O2
IUPAC Name:
2-nitrobenzene-1,4-diamine
Constituent 2
Reference substance name:
2 nitro p phenylene diamine
IUPAC Name:
2 nitro p phenylene diamine
Details on test material:
- Name of test material (as cited in study report):2 nitro p phenylene diamine - Substance type:Organic- Physical state:Solid

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
not specified
Details on test animals or test system and environmental conditions:
TEST ANIMALSSource: No dataAge at study initiation: No dataWeight at study initiation: 1.7-2.5 kg Fasting period before study: No data Housing: No dataDiet (e.g. ad libitum): No dataWater (e.g. ad libitum): No dataAcclimation period: No data

Administration / exposure

Type of coverage:
not specified
Vehicle:
not specified
Details on dermal exposure:
The rabbits were shaved closely the day before the materials were applied in 10 ml of an oxidation-type hair dye base
Duration of exposure:
24 hrs
Doses:
5000 mg/kg (as 10 ml)
No. of animals per sex per dose:
4-8
Control animals:
not specified

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated informationCriteria used for interpretation of results: EU
Conclusions:
The test material was tested for percutaneous toxicity in groups of four to eight New Zealand rabbits (1.7-2.5 kg). The rabbits were shaved closely the day before the materials were applied in 10 ml of an oxidation-type hair dye base. The period of application was 24 hr.The LD50 for the test material in rabbits is > 5000mg/kg
Executive summary:

The test material was tested for percutaneous toxicity in groups of four to eight New Zealand rabbits (1.7-2.5 kg). The rabbits were shaved closely the day before the materials were applied in 10 ml of an oxidation-type hair dye base. The period of application was 24 hr.

 

The LD50 for the test material in rabbits is > 5000mg/kg