Trichloroacetaldehyde

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary literature

Data source

Reference

Reference Type:

secondary source

Title:

Acute Vapor inhalation Toxicity Study for CAS no. 75-87-6

Author:

NTRL report

Year:

1992

Bibliographic source:

NTRL report ,OTS0555527, 1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

Acute inhalation toxicity study of test chemical in albino rats

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trichloroacetaldehyde

EC Number:

200-911-5

EC Name:

Trichloroacetaldehyde

Cas Number:

75-87-6

Molecular formula:

C2HCl3O

IUPAC Name:

2,2,2-trichloroacetaldehyde

Details on test material:

Name of test material (as cited in study report):2, 2, 2-trichloroacetaldehyde
- Molecular formula :C2HCI3O
- Molecular weight :147.387 g/mole
- Substance type:organic
- Physical state:Liquid
Purity:No data available
- Impurities (identity and concentrations):No data available

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

Details on test animal
TEST ANIMALS
- Source: No data available
- Age at study initiation: Specific age was not mention young rats were taken
- Weight at study initiation: 144 grams
- Fasting period before study: No data available
- Housing: in a stock cages
- Diet (e.g. ad libitum): standard laboratory diet (Purina Rat Chow)ad libitum
- Water (e.g. ad libitum): water ad libitum
- Acclimation period: No data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25°C
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available

IN-LIFE DATES: From: To: No data available

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

clean air

Remark on MMAD/GSD:

No data available

Details on inhalation exposure:

No data available

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Remarks on duration:

total : 240min

Concentrations:

0.24,0.36,0.38,0.71,6.32 mg/l

No. of animals per sex per dose:

Total :50
0.24mg/l :10
0.36mg/l:10
0.38mg/l:10
0.71mg/l:10
6.32mg/l:10

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Weighing: Before and after inhalation
- Necropsy of survivors performed: yes
- Other examinations performed:
clinical signs: Yes
body weight: Yes, Before and after 14 days of observation
organ weights: yes
histopathology: No data available
other:

Statistics:

Litchfield and Wilcoxon

Results and discussion

Preliminary study:

No data available

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

0.44 mg/L air

Based on:

test mat.

95% CL:

> 0.38 - < 0.5

Exp. duration:

240 min

Remarks on result:

other: mortality was observed

Mortality:

Mortality was observed at dose 0.36 and 0.38 at four to five days of exposure and at dose 0.71 and 6.32 death was observed at one to three days.

Clinical signs:

other: Sneezing was noted approximately one-half hour after the start of the inhalation period. Hypoactivity, ptosis, and dyspnea occurred at about one hour, with gasping and prostration at two to three hours.

Body weight:

At dose 0.24,0.36 and 0.38 body weight was observed as 70, 39 and 58 grams all are within the normal limits

Gross pathology:

Moderate to severe diffuse red discoloration of the lungs were seen. Three rats from Group 2 did not reveal any gross pathologic alteration

Other findings:

No data available

Any other information on results incl. tables

Acute Vapour Inhalation Toxicity study- Albino Rats

                              Mortality Data

Group	Average. Nominal Vapour Concentration	Number of Animals tested	Observed precent death	Expected percent death
I	0.24	10	0	0.01
II	0.36	10	10	12.10
III	0.38	10	20	20.00
VI	0.71	10	100	99.86
V	6.32	10	100	100

Acute Vapour Inhalation LC50 = 0.44mg,/L air (four-hour exposure)

LimitsofLC50 at the 95 %Levelofconfidence=0.38 – 0.50mg/L air

 

Applicant's summary and conclusion

Interpretation of results:

Category 1 based on GHS criteria

Conclusions:

The LC50 value was considered to be 0.44 mg/l of air (vapour) .When rats were inhaled with the given test chemical.

Executive summary:

Acute inhalation toxicity test was conducted in male rats of Sprague-Dawley strain. 5 groups of 10 rats were taken for test. Rats were exposed for 4 hrs and observed for 14 days. Body weight was measured before and after 14 days of observation. Gross pathology was conducted for the animal which died during test and after completion of observation period. At dose 0.24,0.36 and 0.38 body weight was observed as 70, 39 and 58 grams all are within the normal limits. Moderate to severe diffuse red discoloration of the lungs were seen. Mortality was observed at  dose 0.36 and 0.38 at four to five days of exposure and at dose 0.71 and 6.32 death was observed at one to three days. Therefore, the LC50  was considered to be 0.44 mg/l of air (vapour) .When rats were inhaled with the given test chemical.