2-methylanthraquinone

Endpoint summary

Description of key information

Short-term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical .The studies are as mentioned below:

For the structurally similar read across substance , short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio.The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed.

 Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l.48 h of incubation. Sublethal concentration (LC50) values were calculated by the trimmed Spearman}Karber method.The effect concentration (LC50)of test material on Ciprinus carpio after 48 h was observed to be 0.045 mg/l.

In another study for structurally similar read across substance,short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio. The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed. Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l. After 48 h of incubation Sublethal concentration (LC50) values were calculated by the trimmed Spearman} Karber method.The effect concentration (LC50) of test material on Ciprinus carpio after 48 h was observed to be 0.022 mg/l.

Based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 1 g/l was prepared by dissolving yellow green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 0, 0.03, 0.05, 0.10, 0.20, 0.40 and 0.80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.14 mg/L on the basis of mobility inhibition effects in ay 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 1 g/l was prepared by dissolving yellow-green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of test chemical the ErC50 was determine to be 0.21 mg/l. Thus the ErC50 value, indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute 1/ chronic 1 category as per the CLP criteria.

Toxicity to microorganisms:

Effect of test material was evaluated on microorganism based on the data from peer reviewed journal ,fungal growth inhibition determination of test material was carried out by microplate plate. The test substance was dissolved in dimethyl sulphoxide. The test organism used was fungal species i.e. Aspergillus niger N402. The fungal species was incubated with title compound at 37 deg C for 40 h and growth inhibition was determined by measuring at 590 nm. The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l.

In another data , the Minimal inhibitory concentration (MIC) value of the test substance to the fungal species Candida dubliniensis ATCC 777 was determined to be > 100 mg/ml.

 

Additional information

Short-term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical .The studies are as mentioned below:

For the structurally similar read across substance , short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio.The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed.

 Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l.48 h of incubation. Sublethal concentration (LC50) values were calculated by the trimmed Spearman}Karber method.The effect concentration (LC50)of test material on Ciprinus carpio after 48 h was observed to be 0.045 mg/l.

In another study for structurally similar read across substance,short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio. The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed. Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l. After 48 h of incubation Sublethal concentration (LC50) values were calculated by the trimmed Spearman} Karber method.The effect concentration (LC50) of test material on Ciprinus carpio after 48 h was observed to be 0.022 mg/l.

Based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 1 g/l was prepared by dissolving yellow green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. 0, 0, 0.03, 0.05, 0.10, 0.20, 0.40 and 0.80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 0.14 mg/L on the basis of mobility inhibition effects in ay 48 hour study. Thus the EC50 value, indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic acute 1 / chronic 1 category as per the CLP criteria.

 

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 1 g/l was prepared by dissolving yellow-green powder in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of test chemical the ErC50 was determine to be 0.21 mg/l. Thus the ErC50 value, indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute 1/ chronic 1 category as per the CLP criteria.

Toxicity to microorganisms:

Effect of test material was evaluated on microorganism based on the data from peer reviewed journal ,fungal growth inhibition determination of test material was carried out by microplate plate. The test substance was dissolved in dimethyl sulphoxide. The test organism used was fungal species i.e. Aspergillus niger N402. The fungal species was incubated with title compound at 37 deg C for 40 h and growth inhibition was determined by measuring at 590 nm. The Minimal inhibitory concentration (MIC) value of the test substance to the fungi Aspergillus niger N402 was determined to be 25 mg/l.

In another data , the Minimal inhibitory concentration (MIC) value of the test substance to the fungal species Candida dubliniensis ATCC 777 was determined to be > 100 mg/ml.