2-methylanthraquinone

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
2. and 3. To evaluate short term toxicity of test material on fish Ciprinus carpio

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Cyprinus carpio

Details on test organisms:

2.- Source: Lu-Kan Fish Culture Station, Taiwan Fisheries Research Institute, Taichung, Taiwan

- Length at study initiation: 2 – 6 cm

- Food type: yeast
- Acclimation period: 2 weeks
- Acclimation conditions : acclimatized in 224 (diameter)46 (height)-cm circular plastic pool

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

215 mg/L as CaCO3

Test temperature:

25±1°C

pH:

6.6

Dissolved oxygen:

4.9 mg/L

Nominal and measured concentrations:

2) 10, 50, 100, 200, and 500 mg/l

Details on test conditions:

2) - Test vessel: 10-L beaker
- fill volume: 5L
- No. of organisms per vessel: 4 carp each for 5 different concentration

-For each test, 90% of the incubation medium was renewed with same
concentration of test chemical after 48 h of incubation

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.045 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

2) (LC50) values were calculated by the trimmed Spearman}Karber method

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical is not likely to be toxic to fish atleast in the dose range of 0.022-0.045 mg/l

Executive summary:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical .The studies are as mentioned below:

For the structurally similar read across substance , short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio.The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed.

 Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l.48 h of incubation. Sublethal concentration (LC50) values were calculated by the trimmed Spearman}Karber method.The effect concentration (LC50)of test material on Ciprinus carpio after 48 h was observed to be 0.045 mg/l.

In another study for structurally similar read across substance,short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio. The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed. Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l. After 48 h of incubation Sublethal concentration (LC50) values were calculated by the trimmed Spearman} Karber method.The effect concentration (LC50) of test material on Ciprinus carpio after 48 h was observed to be 0.022 mg/l.

Based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.

 

Description of key information

Short-term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical .The studies are as mentioned below:

For the structurally similar read across substance , short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio.The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed.

 Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l.48 h of incubation. Sublethal concentration (LC50) values were calculated by the trimmed Spearman}Karber method.The effect concentration (LC50)of test material on Ciprinus carpio after 48 h was observed to be 0.045 mg/l.

In another study for structurally similar read across substance,short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio. The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed. Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l. After 48 h of incubation Sublethal concentration (LC50) values were calculated by the trimmed Spearman} Karber method.The effect concentration (LC50) of test material on Ciprinus carpio after 48 h was observed to be 0.022 mg/l.

Based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.

 

Additional information

Short-term toxicity to fish:

Data available for the structurally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical .The studies are as mentioned below:

For the structurally similar read across substance , short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio.The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed.

 Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l.48 h of incubation. Sublethal concentration (LC50) values were calculated by the trimmed Spearman}Karber method.The effect concentration (LC50)of test material on Ciprinus carpio after 48 h was observed to be 0.045 mg/l.

In another study for structurally similar read across substance,short term toxicity test was performed to evaluate the effect of test material on fish Ciprinus carpio. The test animals were fed with yeast in a 224 (diameter)46 (height)-cm circular plastic pool. Before the experiment, the test organisms were acclimatized in aquaria for 2 weeks under conditions similar to those under which the tests were performed. Four carp 2 to 6 cm in size, were introduced to each 10-L beaker containing 5 L of different concentrations of test chemical. Five different concentrations were 10, 50, 100, 200, and 500 mg/l. After 48 h of incubation Sublethal concentration (LC50) values were calculated by the trimmed Spearman} Karber method.The effect concentration (LC50) of test material on Ciprinus carpio after 48 h was observed to be 0.022 mg/l.

Based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1/ chronic 1 as per the CLP classification criteria.