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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test item attained 0 % biodegradation after 28 days and therefore cannot be considered to be readily biodegradable (OECD 301 B and EU Method C.4-C).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
GUIDELINE
A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method was designed to be compatible with OECD Guidelines for Testing of Chemicals (1992) No 301B "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4 -C of Commission Regulation (EC) No 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph m).
METHODS
An initial experiment was conducted at a concentration of 10 mg carbon/L. The toxicity control vessel, containing both the test item and sodium benzoate, attained less than 25 % biodegradation after 14 days. These results indicated that, under the strict terms and conditions of the OECD guidelines, the test item would be classed as exhibiting inhibitory effects.
Therefore, following the recommendations of the test guidelines, in the definitive test, the test item, at a reduced concentration of 5 mg carbon/L was exposed to activated sewage sludge microorganisms with mineral medium in sealed culture vessels in the dark at temperatures of between 19 and 22°C for 28 days.
The biodegradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.
RESULTS
The test item attained 0 % biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301 B. It is also clear from the test item results that some inhibition of the activated sewage sludge microorganisms had occurred as the CO2 evolution rates in the test item vessels were lower than those in the control vessel after 28 days. Care should therefore be taken in the interpretation of the results due to the inhibitory nature of the test item to the activated sewage sludge microorganisms used in the study.
CONCLUSION
The test item attained 0 % biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301 B.
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