Benzyl isobutyrate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Toxicity to micro organism test was performed on Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. to determine the Minimum Inhibitory Concentration(MIC)

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

Sampling method: Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

Vehicle:

yes

Details on test solutions:

- Chemical name of vehicle (organic solvent, emulsifier or dispersant):dimethyl sulfoxide (DMSO)

Test organisms (species):

other: Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC))

Test type:

not specified

Water media type:

not specified

Total exposure duration:

24 h

Hardness:

No data

Test temperature:

No data

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

Test vessel: Agar plates
No. of organisms per vessel: microbial concentration of 106CFU/ml

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

other: Minimum Inhibitory Concentration(MIC)

Effect conc.:

> 2 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

not specified

Validity criteria fulfilled:

not specified

Conclusions:

The Minimum Inhibitory Concentration of test substance Benzyl isobutyrate for Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure.

Executive summary:

 Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 10⁶CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

 

The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE), Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to test chemical.

Description of key information

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE), Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to test chemical.

Key value for chemical safety assessment

EC50 for microorganisms:

2 000 mg/L

Additional information

Various studies available for the test chemical and similar read across chemicals were reviewed to determine the toxic nature of test chemical on the growth and other activity of microorganisms. The studies are as mentioned below:

 

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE), Escherichia coli (ATCC 11775, (EC)) was determine to be > 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to test chemical.

 

The Minimum Inhibition (MIC) effect of test chemical was observed on Corynebacterium minutissimum (CM), Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of test chemical on Corynebacterium minutissimum (CM), Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)), Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) microorganisms species was determine to be  >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure with test chemical.