2,6,10-trimethylundec-9-enal

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 May 2014 - 16 May 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

sewage sludge

Specific details on test material used for the study:

- Lot No.: SC00010875
- Expiry Date: 9 April, 2015

- Trade name: ADOXAL

Details on study design: HPLC method:

A set of six reference substances; acetanilide, methyl benzoate, naphthalene, 1,2,3 trichlorobenzene, phenanthrene and DDT, were used to calibrate the chromatography system. Individual standard solutions of each were prepared along with a mixed standard containing all six reference substances and formamide. In addition, a solution of formamide was also prepared, to be used as the void volume marker, in order to determine the system dead time.

Two series of injections were made, each consisting of duplicate injections of the test substance, duplicate injections of the reference substances and triplicate injections of formamide (the void volume marker). Injections were made in the following order:
Formamide, Acetanilide standard, Methyl benzoate standard, Naphthalene standard, Test substance, Mixed standard, Test substance, 1,2,3-Trichlorobenzene standard, Phenanthrene standard, DDT standard and Formamide

Analytical monitoring:

yes

Details on sampling:

The reference substances, formamide, and test substance stock solutions were prepared in methanol and these were then diluted with 55:45 v/v methanol:double-distilled water diluting solvent (methanol, 110 mL mixed with double-distilled water, 90 mL) to produce working solutions.

Key result

Type:

log Koc

Value:

3.86 dimensionless

pH:

7

Temp.:

25 °C

Remarks on result:

other: Peak 3 (major)

Remarks:

mean of two tests

Details on results (HPLC method):

There was good correlation with all the calibration substances for the Log K vs Log Koc graph, with correlation coefficients of 0.9906 in both tests.
Three peaks were attributable to Adoxal, with adsorprtion coefficients of 3.32, 3.57 and 3.86. From the chromatograms, the latter is the major peak. The smaller peaks may actually be those of the impurities present in Adoxal. The test item had a purity of 90.2%.

Testing was performed at 25°C (column temperature), however the values can be considered to represent the values at an unspecified, probably ambient temperature since no mention is made in the guidelines of the temperature at which the tabulated reference substances log Koc values were determined. Adsoprtion coefficients of the test and reference substances are assumed to vary with temperature in the same way.

Transformation products:

not measured

Remarks:

Not applicable to HPLC estimation method.

Validity criteria fulfilled:

yes

Conclusions:

Three adsorption coefficients were observed corresponding to mean log Koc values of 3.32, 3.57 and 3.86. The latter corresponds to the major peak in the HPLC chromatograms and thus is attributed to the main constituent of Adoxal.

Executive summary:

The adsorption coefficient (Koc) on soil and on sewage sludge was estimated using the High Performance Liquid Chromatography Method. Three peaks were attributable to Adoxal with mean log Koc values of 3.32, 3.57 and 3.86. From the chromatograms, peak 3 is the major component. Thus the Log Koc of 3.86, which corresponds to a Koc value of 7244, has been chosen as the key value for chemical safety assessment.

Description of key information

The adsorption coefficient (Koc) on soil and on sewage sludge was estimated using the High Performance Liquid Chromatography Method. Three peaks were attributable to Adoxal with mean log Koc values of 3.32, 3.57 and 3.86. From the chromatograms, peak 3 is the major component. Thus the Log Koc of 3.86, which corresponds to a Koc value of 7244, has been chosen as the key value for chemical safety assessment.

Key value for chemical safety assessment

Koc at 20 °C:

7 244

Additional information

Testing was performed at 25°C (column temperature), however the values can be considered to represent the values at an unspecified, probably ambient temperature since no mention is made in the guidelines of the temperature at which the tabulated reference substances log Koc values were determined. Adsorption coefficients of the test and reference substances are assumed to vary with temperature in the same way.

[LogKoc: 3.86]