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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
19-05-2017 to 22-05-2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
Aim of this study was to evaluate the nature of chemical when comes in contact with the test organism. Test was conducted according to the OECD guideline 201.
GLP compliance:
no
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
The stock solution 100.0 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Test temperature:
23±2°C
pH:
control : pH = 8.1 changed to pH = 8.8 during the test
Nominal and measured concentrations:
20, 30, 44 , 66, 100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed
- Sample volume: 15 ml
- Initial cells density: 9.8x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
83.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95 % CI 18.6 - 84.7 mg/l
Remarks:
Tested substance is an acid and that is why the higher concentration the lower pH. The optimum pH for algae is about 8.0. The highest concentration could be influenced by lower pH. Adjustment of pH would change substance properties.
Details on results:
Tested substance is an acid and that is why the higher concentration the lower pH. The optimum pH for algae is about 8.0. The highest concentration could be influenced by lower pH. Adjustment of pH would change substance properties.
Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.75 mg/L (24 hours)
Reported statistics and error estimates:
EC50 was calculated using non linear regression by the software Prism 4.0
Validity criteria fulfilled:
yes
Conclusions:
Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical the EC50 was determine to be 83.1 mg/l.
Executive summary:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100.0 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The nominal concentration were selected as 20, 30, 44 , 66, 100 mg/l . Tested substance is an acid and that is why the higher concentration the lower pH. The optimum pH for algae is about 8.0. The highest concentration could be influenced by lower pH. Adjustment of pH would change substance properties.The median effective concentration (EC50) for the test substance, in algae was determined to be 83.1mg/L on the basis of growth rate inhibition effects in a 72 hour study.The test material can not be classified based on the above effect concentration as the inhibition of the mobility of daphnids could be influenced by pH.

Description of key information

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 100.0 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The nominal concentration were selected as20, 30, 44 , 66, 100 mg/l . Tested substance is an acid and that is why the higher concentration the lower pH. The optimum pH for algae is about 8.0. The highest concentration could be influenced by lower pH. Adjustment of pH would change substance properties.The median effective concentration (EC50) for the test substance, in algae was determined to be 83.1mg/L on the basis of growth rate inhibition effects in a 72 hour study.The test material can not be classified based on the above effect concentration as the inhibition of the mobility of daphnids could be influenced by pH.

Key value for chemical safety assessment

Additional information

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The stock solution 100.0 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The nominal concentration were selected as20, 30, 44 , 66, 100 mg/l . Tested substance is an acid and that is why the higher concentration the lower pH. The optimum pH for algae is about 8.0. The highest concentration could be influenced by lower pH. Adjustment of pH would change substance properties.The median effective concentration (EC50) for the test substance, in algae was determined to be 83.1mg/L on the basis of growth rate inhibition effects in a 72 hour study.The test material can not be classified based on the above effect concentration as the inhibition of the mobility of daphnids could be influenced by pH.