1-chloro-2,5-dimethoxybenzene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1976-10-13 to 1976-10-27

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Insufficient description of effect doses.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: company guideline

Deviations:

not applicable

Principles of method if other than guideline:

Acute inhalation toxicity of the liquid test substance was tested in the inhalation risk assay. Six male SPF-Wistar-rats served as testing animals. The mean body weight was 121g (ranging from 115-124g). Exposure takes place at a room temperature of 23.3°C and a barometric pressure of 738.4Torr. Animal were placed in a glass vessel of 39L and exposed to the generated mixture of air and the gaseous substance. The exposure time was 8hrs.

On order to generate a mixture of air and the gaseous test substance the testing compound was transferred into a gas wash bottle equipped with a glass frit. Before and after testing the bottle was weighed. Using a rotameter the airflow through the gas wash bottle was adjusted to 400L per h.

During inhalation and 14d thereafter the behaviour of the test animals was observed. During this time the body weight was determined on a daily basis. Within the observation period access to food (Altromin 1324, Company Altromin GmbH, Lage/Lippe) and tap water was ad libitum. Housing of the rats were plastic cages filled with wood shavings. At the end of the observation period all animals were euthanised after narcotization, and than dissected and macroscopically evaluated.

GLP compliance:

no

Remarks:

- Performed prior to GLP implementation

Test type:

other: inhalation risk assay

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2,5-dimethoxybenzene

EC Number:

218-267-9

EC Name:

1-chloro-2,5-dimethoxybenzene

Cas Number:

2100-42-7

Molecular formula:

C8H9ClO2

IUPAC Name:

2-chloro-1,4-dimethoxybenzene

Details on test material:

- Name of test material (as cited in study report): CME = Chlorhydrochinondimethyläther (2-Chlor-1,4-dimethoxybenzol)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

- Ambient temperature: 23.3°C
- Barometric pressure: 738,4 Torr
- Housing: Plastic cages filled with wood shavings
- Food and tap water: ad libitum, food (ALtromina 1324) supplier: Altromina GmbH, Lage/Lippe
- Mean body weight: 121g, ranging from 115-124g

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

other: air

Details on inhalation exposure:

- Exposure time was one time for 8hrs
- The testing compound was transferred to a gas wash bottle equipped with a glass frit
- Before and after testing the bottle was weighed
- Using a rotameter the airflow was adjusted to 400L per h
- During inhalation and 14d thereafter behaviour of the test animals was observed

Analytical verification of test atmosphere concentrations:

yes

Remarks:

gravimetric determination of the substance mass spent for generation of exposure atmosphere

Duration of exposure:

8 h

Concentrations:

see table 1

No. of animals per sex per dose:

6 male Wistar-rats

Control animals:

no

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

discriminating conc.

Effect level:

ca. 600 other: mg of gaseous test substance within 8hrs of exposure. Air flow was 400L per h of a mixture of air and the gaseous test substance. Volume of the exposure chamber (galss veesel) was 39L.

Exp. duration:

8 h

Remarks on result:

other: No lethality

Mortality:

none

Clinical signs:

other: - 3mins after beginn of exposure: restlessness - 6mins after beginn of exposure: increased breathing rate, prone position - 36mins after beginn exposure: closed eyes, squatting posture, increased breathing rate - 1d after treatment: slightly increase bre

Body weight:

normal body weight gain

Gross pathology:

dirty red coloured lungs

Applicant's summary and conclusion

Interpretation of results:

other: Not classified. Insufficient description of effect doses. No relevant data that can be compared to classification criteria.

Remarks:

Criteria used for interpretation of results: expert judgment

Conclusions:

No lethality at the concentration tested.

Executive summary:

In the present inhalation toxicity study all animals survived a single 8hrs exposure to CME. During inhalation the following symptoms were observed: restlessness, increased breathing rate, closed eyes. Dissection revealed a dirty red coloration of the lung.