1-butyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1977

Materials and methods

Principles of method if other than guideline:

- Acute intraperitoneal toxicity testing (mouse) according to BASF-internal standard:
In principle, the methods described in OECD Guideline 401 were used, except that the substance preparations were injected into the peritoneal cavity of mice.
Young adult laboratory mice were purchased from a breeder. Several groups of 5 to 10 mice per sex and dose were treated simultaneously by injecting preparations of the test substance in a suitable vehicle. Group-wise documentation of clinical signs was performed over the 7- to 14- day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose.The clinical signs and findings were reported in summary form. More details e.g. on substance preparation, or dose and time dependence of symptoms, can be inferred from the German hand written raw data.On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-butyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

EC Number:

254-296-3

EC Name:

1-butyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

Cas Number:

39108-47-9

Molecular formula:

C11H14N2O2

IUPAC Name:

1-butyl-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile

Details on test material:

Name of the test substance used in the study report: N-Butyl-2-hydroxy-3-cyan-3-methylpyridon(6)

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

Mean body weight: males 22 g, females 21 g
A standardized animal laboratory diet was used.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

The test concentrations used were 4.64, 6.81, 10, 14.7, 21.5 and 31.6% (G/V); suspension in 0.5% aqueous CMC.

Doses:

464, 681, 100, 1470, 2150 and 3160 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: ALD50

Effect level:

ca. 1 100 mg/kg bw

Mortality:

464 mg/kg: no deaths after 14 days; 681 mg/kg: 1/10 after 14 days; 1000 mg/kg: 2/10 after 14 days; 1470, 2150 and 3160 mg/kg: 10/10 after 14 days

Clinical signs:

dyspnea, apathy, partly abdominal position, staggering, missing pain reflex, twitching, trembling, clonic convulsions, urine red-coloured, exsiccosis, poor general state

Gross pathology:

no intra-abdominal residues and no adherences