Branched and linear octadecanoic acid, esters with glycerol oligomers

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from similar mixture/product

Adequacy of study:

key study

Study period:

25 Jul - 01 Aug 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Leuciscus idus f. melanotus were exposed to the substance in aerated glas test vessels (40 x 25 x 30 cm) containing 20 L test medium under controlled conditions and a 12 h light/dark cycle for 96 h. 65 h prior to exposure begin 10 fish were placed into each test vessel. Then, test substance was added and water quality parameters were measured regularly. The fish were regularly inspected throughout the test. At test end, the fish were harvested, dissected and macroscopically analysed.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the liquid test substance was first dispersed in test medium, then added to the testing vessels and mixed with a glass rod
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
- Evidence of undissolved material: no

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: Ide (German: Goldorfe)
- Source: Paul Eggers, Hohenwestedt, Germany
- Age at study initiation: not specified
- Length at study initiation: 5.5 - 6.1 cm; Mean = 5.9 cm; S.D. = 0.2 cm; n = 10
- Weight at study initiation: 1.4 -2.5 g; Mean = 2.1 g; S.D. = 0.32 g; n = 10

ACCLIMATION
- Acclimation period: 14 d prior beginn of exposure
- Acclimation conditions: not specified
- Type and amount of food during acclimation: Tetra Min (Tetra Werke, Melle, Germany)
- Feeding frequency during acclimation: not specified

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

1.694 mmol/L (9.5 °dH)

Test temperature:

20 ± 1 °C

pH:

8.0 - 8.3

Dissolved oxygen:

> 7 mg/L

Conductivity:

< 5 µS/cm

Nominal and measured concentrations:

nominal: control, 1, 10, 100, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: glass; 40 x 25 x 30 cm; Headspace = 10 L; Fill volume = 20 L
- Aeration: yes, 100 mL air/min
- Renewal rate of test solution: none, static test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): not specified
- No. of vessels per control (replicates): not specified


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised tap water, CHRIST-Ministil-ion exchange cartridge
- Culture medium different from test medium: yes, dechlorinated tap water was used during culture
- Intervals of water quality measurement: before exposure and after 2, 24, 48, 72 and 96 h (pH, temperature, oxygen concentration)

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: 12 h light/12 h dark
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED
Mortality (after 48 h and 96 h); Behaviour (during exposure); Macroscopic assessment of tissue (end of exposure)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5 - 10
- Range finding study: no

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Behavioral observation of fish exposed to 500 mg/L test substance after 24 h: swimming at the surface.
No other behavioral diffences between control and treatment groups were observed.

Results with reference substance (positive control):

- Results with reference substance valid? not applicable

Validity criteria fulfilled:

not applicable

Description of key information

LC50 (96 h) > 500 mg/L (nominal, no guideline followed but comparable to OECD 203); read-across

Additional information

There are no studies available investigating the effects of the target substance Polyaldo 2 -1 -IS (CAS 73296 -86 -3) on freshwater fish. However, there is one study available for the main constituent Di(isooctadecanoic) acid, diester with oxydi(propanediol) (CAS 67938-21-0), which is considered reasonable to cover this endpoint adequately (see read-across justification in IUCLID section 13).

The key study was not performed according to a guideline but the methods are comparable to OECD 203. Leuciscus idus melanotus was exposed to four loading rates in the range of 1 to 500 mg/L (nominal) under static conditions and a 12 h light/dark cycle. The temperature was held at 20 ± 1 °C using a water bath. Mortality and behaviour were recorded at regular intervals and were compared to the negative control at the end of the test.

No mortality was observed after 96 h. Behavioral abnormalities were only observed after 24 h in fish exposed to the highest tested concentration of 500 mg/L, which swam at the water surface. Dissection of the fish after test termination did not reveal any macroscopic changes. The derived LC50 (96 h) was > 500 mg/L.

Based on the available result from one structurally related source substance (in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5) which is a main constituent of the target substance, it can be concluded that the effect concentrations of Reaction product of Isooctadecanoic acid, ester with oxybis[propanediol] (CAS 73296-86-3) will be in the same range.