Trisodium 4-[[4-chloro-6-[(4-sulphonatophenyl)amino]-1,3,5-triazin-2-yl]amino]-2-[[1-(2,5-dichloro-4-sulphonatophenyl)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-4-yl]azo]benzenesulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

In the study the decoloration of textile dye Cibacron Brilliant Yellow 3B-A and their effect on toxicity on Vibrio fischeri are evaluated by Microtox assay.

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC name: trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate
- Name of test material (as cited in study report): Cibacron Brilliant Yellow 3B-A
- Molecular formula : C25H18Cl3N9O10S3.3Na
- Molecular weight : 872.974 g/mol
- Smiles notation : c1(\N=N\c2c(n(c3cc(c(S([O-])(=O)=O)cc3Cl)Cl)nc2C)O)cc(ccc1S(=O)(=O)[O-])Nc1nc(nc(n1)Cl)Nc1ccc(cc1)S(=O)(=O)[O-].[Na+].[Na+].[Na+]
- InChl : 1S/C25H18Cl3N9O10S3.3Na/c1-11-21(22(38)37(36-11)18-9-16(27)20(10-15(18)26)50(45,46)47)35-34-17-8-13(4-7-19(17)49(42,43)44)30-25-32-23(28)31-24(33-25)29-12-2-5-14(6-3-12)48(39,40)41;;;/h2-10,38H,1H3,(H,39,40,41)(H,42,43,44)(H,45,46,47)(H2,29,30,31,32,33);;;/q;3*+1/p-3
- Substance type:Organic
- Physical state: Solid

Analytical monitoring:

yes

Details on sampling:

Eight serial dilutions was used in study.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls:1 g ZnSO4 · 7H2O l−1 was used as the positive control and 1 g glucose l−1 as the negative control.

Test organisms (species):

Vibrio fisheri

Details on inoculum:

Frozen sample was brought to room temperature and centrifuged.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

5 min

Hardness:

No data

Test temperature:

room temperature

pH:

6

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Details on test conditions:

TEST SYSTEM
- Test vessel:Erlenmeyer shake flasks
- No. of vessels per concentration (replicates):triplicate

OTHER TEST CONDITIONS
- Adjustment of pH:The pH of the sample was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH.

Reference substance (positive control):

not specified

Key result

Duration:

5 min

Dose descriptor:

other: EC20

Effect conc.:

> 75 other: %

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: 20% decrease of bioluminescence in the Microtox assay

Remarks on result:

other: EC20 is the percent dilution of the sample (v/v)

Details on results:

EC20 reported is the average of triplicate analysis.

Validity criteria fulfilled:

not specified

Conclusions:

From Microtox assay the test substance trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate i.e Cibacron Brilliant Yellow 3B-A was moderately non-toxic to micro organism Vibrio fischeri on the basis of the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20).

Executive summary:

In the study effect on toxicity on Vibrio fischeri are evaluated by Microtox assay for textile dye Cibacron Brilliant Yellow 3B-A (IUPAC name- trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate).

 

Frozen samples were brought to room temperature,and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples according to the test protocols defined by the manufacturer (Azur Environmental, Newark, Del.).From eight serial dilutions,the percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri (EC20) after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software (1999) Azur Environmental, Newark, Del.]. A solution of 1 g ZnSO4 · 7H2O l−1 was used as the positive control and 1 g glucose l−1 as the negative control. Each EC20 reported is the average of triplicate analysis.

 

As per the criteria (the percent concentration to decrease 20% of the luminescence of Vibrio fischeri EC20))

>100% = not toxic

100%> EC20 > 75% = moderately non-toxic

75% > EC20 > 50% = toxic

50% > EC20 > 25% = moderately toxic

 

And test chemical Cibacron Brilliant Yellow 3B-A gives the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20) was 100%> EC20 > 75% thus it is concluded that the textile dye Cibacron Brilliant Yellow 3B-A was observed to be moderately non-toxic to micro organism Vibrio fischeri.

Description of key information

From Microtox assay the test substance  trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate i.e Cibacron Brilliant Yellow 3B-A was moderately non-toxic to micro organism Vibrio fischeri  on the basis of the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20).

Additional information

In the study effect on toxicity on Vibrio fischeri are evaluated by Microtox assay for textile dye Cibacron Brilliant Yellow 3B-A (IUPAC name- trisodium 2,5-dichloro-4-(4-{[5-({4-chloro-6-[(4-sulfonatophenyl)amino]-1,3,5-triazin-2-yl}amino)-2-sulfonatophenyl]diazenyl}-3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonate).

 

Frozen samples were brought to room temperature,and centrifuged. The pH of the samples was adjusted where necessary to 6 by adding 0.5 ml 0.58 M KH2 PO4 and 70 μl 1 M NaOH. Colour correction was done at 490 nm. The Microtox acute toxicity assay was performed in a Microtox 500 Analyzer on samples according to the test protocols defined by the manufacturer (Azur Environmental, Newark, Del.).From eight serial dilutions,the percent concentration to decrease 20% of the luminescence of amodified strain of Vibrio fischeri (EC20) after 5 min incubation was calculated with the Microtox data analysis program [Microtox Omni Software (1999) Azur Environmental, Newark, Del.]. A solution of 1 g ZnSO4 · 7H2O l−1 was used as the positive control and 1 g glucose l−1 as the negative control. Each EC20 reported is the average of triplicate analysis.

 

As per the criteria (the percent concentration to decrease 20% of the luminescence of Vibrio fischeri EC20))

>100% = not toxic

100%> EC20 > 75% = moderately non-toxic

75% > EC20 > 50% = toxic

50% > EC20 > 25% = moderately toxic

 

And test chemical Cibacron Brilliant Yellow 3B-A gives the percent concentration to decrease 20% of the luminescence of Vibrio fischeri (EC20) was 100%> EC20 > 75% thus it is concluded that the textile dye Cibacron Brilliant Yellow 3B-A was observed to be moderately non-toxic to micro organism Vibrio fischeri.