1-chloro-2,5-dimethoxy-4-nitrobenzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-04-11 to 1988-04-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented GLP and guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Remarks:

GLP compliance statement attached to full study report.

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2,5-dimethoxy-4-nitrobenzene

EC Number:

230-086-7

EC Name:

1-chloro-2,5-dimethoxy-4-nitrobenzene

Cas Number:

6940-53-0

Molecular formula:

C8H8ClNO4

IUPAC Name:

1-chloro-2,5-dimethoxy-4-nitrobenzene

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling at start of study and after 48 and 96 hrs
- Amount: approx. 100 ml

Test solutions

Vehicle:

no

Details on test solutions:

- Preparation of stock solution of 3 g/l
- After filtration test substance concentration 15.4 mg/l
- This solution served as basis for subsequent concentrations

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Source: Hoechst AG, own breed of Department of Gewerbetoxikologie
- Age: hatched 1988-01-15
- Temperature: 22 +/- 1 °C

ACCLIMATISATION
- Oxygen contents: >= 80% of saturation value
- Lighting conditions: 12 hrs daily
- Population density: >= 1 g Fish per L water
- Feedings: twice daily, ad libitum
- Diet: Tetra Min

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

Mean over study period: (°C)
Concentration groups: 21.4 - 22.1
control groups: 21.5 - 21.9

pH:

Mean over study period:
Concentration groups: 7.4 - 8.0
control groups: 7.2 - 8.0

Dissolved oxygen:

Mean over study period: (mg/l)
Concentration groups: 7.9 - 9.1
control groups: 7.5 - 9.0

Nominal and measured concentrations:

Nominal (mg/l) Measured mean (mg/l)
1.93 1.85 96.0 %
3.85 3.37 87.5 %
7.70 6.43 83.5 %
15.40 12.5 81.4 %

Details on test conditions:

- 10 fish per testing or control basin.
- Fasting of fish during entire study period
- Examination of fish at 2-4, 24, 48 and 72 hrs: Signs of lethality, obvious changes in appearance or behaviour
- Dead fish where removed from basins

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

7.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

10.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

15.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

7.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

15.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: swimming and lying on water surface, reduced activity, balance put on tails during swimming, elevated or reduced respiratory frequencey, gill covers sticking out, tumbling.

Reported statistics and error estimates:

Analysis of lethality results by probit analysis not possible. Since factor of concentration levels SQRT(2), no further concentrations tested. Geometric mean of LC0 and LC100 reported.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Conditions over the test period

testconcentration        controls

pH:              7.4 - 8.0                     7.2 - 8.0

O2 [mg/L]:   7.9 - 9.1                     7.5 - 9.0

Temp (°C): 21.4 - 22.1               21.5 - 21.9

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The test of acute toxicity of nitrochlorhydrochinondimethylether in zebra danio (Brachydanio rerio) over a period of 96 hours resulted in the following lethal concentrations:

	48 hours [mg/l]	96 hours [mg/l]
LC0	7.7	7.7
LC50	10.9	10.9
LC100	15.4	15.4

Concentrations tested: 0 / 1.93 / 3.85 / 7.7 and 15.4 mg/l.

At a concentration of 15.4 mg/l symptoms and lethality could be observed. However, the 1.93 up to 7.7 mg/l-groups showed symptoms but no lethalities.

Water samples were taken at the start of the study, after 48 and 96 hours from the middle of the basin to determine the test substance concentration. The mean values are at 81.4 - 96.0 % of the nominal value.