1,4-dichlorobutane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981-09-23

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Acute toxicities of organic chemicals to fathead minnows (pimephales promelas), Vol 1

Author:

Brooke LT, Call DJ, Geiger DL, Northcott CE (eds)

Year:

1984

Bibliographic source:

Center for Lake Superior Studies, University of Wisconsin-Superior. ISBN 0-9614968-0-0

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichlorobutane

EC Number:

203-778-1

EC Name:

1,4-dichlorobutane

Cas Number:

110-56-5

Molecular formula:

C4H8Cl2

IUPAC Name:

1,4-dichlorobutane

Details on test material:

- SMILES notation: CLCCCCCL

Specific details on test material used for the study:

CAS No. 110-56-5
MW: 127.01
Purity: 97%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken at 0 h and 96 h from all test chambers. From one test chamber duplicates were taken at 24 h, 48 h, 72 h and 96 h.

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Source: brood stock from U.S. EPA Environmental Research Laboratory-Duluth
- Age at study initiation (mean and range, SD): 30-31 days
- Weight at study initiation: 0.173 g
- Length at study initiation: 21.6 mm
- Acclimation conditions: Fish were held at 25 °C in flowing water with 16 h light-controlled photoperiod.
- Type and amount of food during acclimation: brine shrimp and dry flake. No feeding during exposure
- Health: Only fish with healthy appearance and no history of abnormally high mortality rate were used in the test.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

44.5 mg/L CaCO3

Test temperature:

24.6 °C

pH:

7.68

Dissolved oxygen:

6.8 mg/L

Salinity:

41.5 mg/L CaCO3

Nominal and measured concentrations:

nominal 0, 25, 42, 69, 116, 193 mg/L. measured average concentrations from two tanks: 7.88 and 9.2 mg/L (25 mg/L nominal); 17.2 and 15.7 mg/L (42 mg/L nominal); 27.9 and 27.0 mg/L (69 mg/L); 48.8 and 52.7 mg/L (116 mg/L nominal); 74.3 and 88.8 mg/L (193 mg/L nominal).

Details on test conditions:

Tank volume 6.3 L, additions 4.7 v/d, 2 replicates per concentration. 25 fish per replicate, loading 0.687 g/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

51.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Conf. limit 47.8 - 55.6 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

51.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Conf. limit 47.8 - 55.6 mg/L

Details on results:

Affected fish became lethargic and stopped schooling. No reference to substance concentration were done.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 for Pimephales promelas after 96 h exposure has been determined to be 51.6 mg/L based on measured concentrations.