2,2'-[(2,2',5,5'-tetrachloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Year of publication: 1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test performed according to conditions as detailed in OECD 209. However, information on the test item is limited.

Data source

Reference

Reference Type:

publication

Title:

THE ASSESSMENT OF THE POSSIBLE INHIBITORY EFFECT OF DYE STUFFS ON AEROBIC WASTE-WATER BACTERIA EXPERIENCE WITH A SCREENING TEST

Author:

Brown, D; Hitz, HR; Schäfer, L

Year:

1981

Bibliographic source:

Chemosphere, Vol. 10, No. 3, pp 245 - 261, 1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

EC Number:

225-822-9

EC Name:

2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

Cas Number:

5102-83-0

Molecular formula:

C36H34Cl2N6O4

IUPAC Name:

2,2'-[(3,3'-dichlorobiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis[N-(2,4-dimethylphenyl)-3-oxobutanamide]

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

The sludge used by the different laboratories (5 laboratories) was domestic for 4 laboratories. One laboratory however used industrial sludge.
Preparation and amount of sludge used was as specified by OECD 209.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test conditions

Test temperature:

20 +- 2°C

pH:

pH: between 7 and 8

Nominal and measured concentrations:

Limit test: 100 mg/L nominal concentration

Details on test conditions:

Identical to OECD-guideline 209, as this publication was the basis for the development of the guideline.
For those chemicals whose respiration rate was at least 80% of the control, the IC50 (concentration for 50% inhibition of respiration rate) is recorded as >100 mg/L.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The respiration rate for the submission substance at a concentration of 100 mg/L was >= 80% of the control samples without test item.

Results with reference substance (positive control):

Concentration of 3,5-dichlorophenol [mg/L] :: mean inhibition [%]:

100 :: 90%
50 :: 91%
25 :: 78%
10 :: 36%
1 :: 6%

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Remarks:

However, as validity criteria for guideline OECD 209 were specified, these criteria were probably fulfilled for the reported test results.

Conclusions:

In a publication regarding the development of a screening test for inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later used as the methodical basis of the OECD 209 activated sludge respriation inhibition test, the toxicity of the submission substance to aerobic waste water bacteria had been tested. The EC50 (3 hours) had been determined as > 100 mg/L (defined as at least 80% respiration rate of the control) . This is probably a nominal concentration, however no specific indication is given. The sludge used by the different laboratories was predominantly domestic (4 laboratories used domestic, one of five laboratories however used industrial sludge). It is not clear, which type of sludge had been used with the submission substance. No information on substance purity is given.
However, as this interlaboratory study formed the basis for OECD guideline 209, the data are judged to be reliable (reliability category 2). From these data, the submission substance would be regarded as not harmful to aerobic microorganisms of sewage treatment plants.

Executive summary:

In a publication regarding the development of a screening test for inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later used as the methodical basis of the OECD 209 activated sludge respriation inhibition test, the toxicity of the submission substance to aerobic waste water bacteria had been tested. The EC50 (3 hours) had been determined as > 100 mg/L (defined as at least 80% respiration rate of the control) . This is probably a nominal concentration, however no specific indication is given. The sludge used by the different laboratories was predominantly domestic (4 laboratories used domestic, one of five laboratories however used industrial sludge). It is not clear, which type of sludge had been used with the submission substance. No information on substance purity is given.

However, as this interlaboratory study formed the basis for OECD guideline 209, the data are judged to be reliable (reliability category 2). From these data, the submission substance would be regarded as not harmful to aerobic microorganisms of sewage treatment plants.