Tin bis(2-ethylhexanoate)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Very brief description of methods and results. Characterization of test material was not reported. No pathological examinations of the test animals were reported.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Reference 2

Reference Type:

publication

Title:

Final Submittal: Tin bis(2-Ethylhexanoate) CAS Number 301-10-0

Author:

The Metal Carboxylates Coalition

Year:

2007

Bibliographic source:

U.S. High Production Volume (HPV) Chemical Challenge Program

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method: The test substance was placed in a syringe and introduced through the esophagus into the stomach with a stainless steel catheter.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tin bis(2-ethylhexanoate)

EC Number:

206-108-6

EC Name:

Tin bis(2-ethylhexanoate)

Cas Number:

301-10-0

Molecular formula:

C16H30O4Sn

IUPAC Name:

tin bis(2-ethylhexanoate)

Details on test material:

Tin (II) Ethylhexanoate [CAS No. 301-10-0]

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: No data
- Age at study initiation: Young adult
- Weight at study initiation: 200-250 grams
- Fasting period before study: Fasted 24 hours prior to dosing.
- Housing: Cages with wire mesh floors elevated above dropings in groups of 5.
- Diet : ad libitum
- Water : ad libitum
- Acclimation period: No data

ENVIRONMENTAL CONDITIONS
- Temperature: 72 deg F
- Humidity: No data
- Air changes: No data
- Photoperiod (hrs dark / hrs light): Light was furnished for eith out of every tewnty-four hour period.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Remarks:

none

Details on oral exposure:

no vehicle

Doses:

1.6, 3.2, 6.4 and 12.8 g/kg

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 21 days
- Frequency of observations and weighing: No data
- Necropsy of survivors performed: No data
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: Animals were observed for 21 days.

Statistics:

The LD50 was calculated using the Thompson Moving Average Method (Biometrics, September, 1952, Vol. 8, No. 3).

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

5 870 mg/kg bw

95% CL:

>= 3.14 - <= 10.98

Remarks on result:

other: reported in g/kg

Mortality:

1.6 g/kg - 0 mortality
3.2 g/kg - one mortality on day 12 of the test
6.4 g/kg - three mortalities on day 2 of the test
12.8 g/kg - one mortality on day 2 and thee mortalities on day 4 of the test

Clinical signs:

other: The rats which received the two higher doses, 6.4 and 12.8 g/kg were listless following administration of the test material.

Gross pathology:

No data

Other findings:

No data

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The oral LD50 for tin bis(2 -ethylhexanoate) is 5.87 g/kg with 95% confidence limits of 3.14 g/kg to 10.98 g/kg.

Executive summary:

An approximation of the LD50 was attained by administering the chemical compound to a number of rats on each of several levels. A group of twenty young adult male albino rats of the Sprague-Dawley Strain weighing approximately 200 -250 grams was selected for use in this study. The animals were divided into four subgroups of five animals each and fasted for twenty-four hours prior to dosing. The experimental material was placed in a syringe and introduced through the esophagus into the stomach with a stainless steel catheter. Five rats were dosed at 1.6, 3.2, 6.4 and 12.8 g/kg with the test materail as supplied. Animals on the same dosage level were then placed in a common cage with free access to food and water. The cages employed had wire mesh floors elevated abouve the droppings and were kept in temperature controlled rooms at 72 deg F +/- 2 deg F. Light was furnished for eight out of every twenty-four hour period. The animals were observed for a twenty-one day period and deaths were recorded. The LD50 was calculated using the Thompson Moving Average Method (Biometrics, September, 1952, Vol. 8, No. 3). Rats which received the two higher doses were listless following administration of compound. Fatalities occurred during the first four days on these two levels. One death occurred on the twelfth day at 3.2 g/kg level. The oral LD50 of tin bis(2 -ethylhexanoate) is 5.87 g/kg with 95% confidence limits of 3.14 g/kg to 10.98 g/kg.