Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental result using standard method of analysis

Justification for type of information:

Data is from experimental reports.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Short term toxicity to fish tests was carried out according to the OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt
- Molecular formula (if other than submission substance): C27H31N2O6S2.Na
- Molecular weight (if other than submission substance): 566.672g/mol
- Substance type: Organic
- Physical state:Solid

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Animal species: Zebra Fish (Danio rerio)
Animal breeder/ supplier: Asim Fancy Fish Aquarium, Dharampeth
Body length: Average 2.46 cm
Body weight: Average 0.094 g

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

81.5 (mg of CaCO3)

Test temperature:

25oC

pH:

7.7

Salinity:

1175 TDS (ppm)
513 Conductivity (µScm-1)

Nominal and measured concentrations:

6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L (Nominal)

Details on test conditions:

Photoperiod: 12 hours light and 12-hour darkness
Temperature range: 24.5°C
pH range: 7.9
DO range: 7.9mg/L
Feeding: Once in a day up to sixth day of acclimatization
Aeration: Continuous aeration was provided
Mortality (%) During last 48 hours = 3%

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not toxic

Sublethal observations / clinical signs:

A 96 h study was conducted for CAS No.129-17-9 on Zebra Fish (Danio rerio), at a test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L mg/L. No mortalities were found in the control aquaria. 

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100% mortality was not observed in any of the concentrations

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 129-17-9 on Zebra Fish (Danio rerio) was found to be >100 mg/L I t can be concluded from the value that the Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt is not toxic to the aquatic evironment.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L andZebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L. I t can be concluded from the value that the Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt is not toxic to the  aquatic evironment amd can be considered as "not classified" as per the classification criteria

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L andZebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.I t can be concluded from the value that the Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt is not toxic to the  aquatic evironment .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Following experimental study from ABITEC for structurally similar read across Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] die...; sodium 4-{[4-(diethylamino)phenyl][4-(diethyliminio)cyclohexa-2,5-dien-1-ylidene]methyl}benzene-1,3-... (CAS: 129-17-9) suggests that the Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L andZebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.I t can be concluded from the value that the Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt is not toxic to the  aquatic evironment amd can be considered as "not classified" as per the classification criteria.

It can be concluded from the above expiemental study that the Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, aluminium salt (CAS:15876-40-1) not toxic to the aquatic environment.