Biphenyl-2-ol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study according to national standards

Qualifier:

according to guideline

Guideline:

other: Procedures recommended by the ASTM Subcommittee on Safety to Aquatic toxicology: ASTM Standard E729-80, Standard Practice for Conducting Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians, Philadelphia, PA, 1980.

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0.5, 1.0., 10 mg a.i./L
- Sampling time: 0, 48 h

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test concentrations were prepared by adding appropriate amounts of 0.2 mg/mL aqueous stock solution of the test material in daphnid dilution water to test vessels containing approximately 100 mL of dilution water and bringing the final volume up to 200 mL with additional dilution water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain: Daphnia magna STRAUS
- Source: Laboratory culture
- Age at study initiation: Juvenile Daphnia magna 1st instar
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

148 mg/L as CaCO3

Test temperature:

19.7 - 21.0 °C

pH:

7.9 - 8.1

Dissolved oxygen:

O2 saturation: > 95%

Nominal and measured concentrations:

Nominal test concentrations: control, 0.78, 1.3, 2.2, 3.6, 6.0, 10.0 mg/L
Recovery rates: 92 - 100% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: Pyrex beakers, 250 ml, 200 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: standard dilution water; pumped from the upper Saginaw Bay of Lake Huron of Whitestone Point. The water is carbon filtered, UV irradiated and pH adjusted with CO2 in the laboratory prior to use. In addition to the above, the most recent analysis of selected organics, pesticides and PCBs or both dilution waters indicated acceptably low levels.
- Alkalinity: 45 mg/L
- Ca/Mg ratio: 38250 µg/L / 7670 µg/L
- Conductivity: 330 µS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16h/8h light dark cycle

EFFECT PARAMETERS MEASURED: Immobility after 24 and 48 hours

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.78 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

- Mortality of control: < 10% after 48 h

Executive summary:

The study from Dill (1985) was conducted according to ASTM Standard E729-80, Standard Practice for Conducting Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians and revealed a LC50 of 2.7 mg/L for Daphnia magna.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

18-20 °C

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.71 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 2.34-3.17 mg/L

Validity criteria fulfilled:

not specified

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study according to a national standard method

Qualifier:

according to guideline

Guideline:

other: DIN-Standard 38 412 L11 (Daphnia, Short-time toxicity test): method of the German Standards Institution, Berlin, Germany

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Description of key information

LC50 (48 h) for Daphnia magna = 2.7 mg a.i./L (nominal, ASTM Guideline (Standard E729-80)) 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.7 mg/L

Additional information

For the assessment of the toxicity of OPP (CAS-No. 90-43-7) on aquatic invertebrates two Guideline studies are available.

In the key study individuals of Daphnia magna were exposed to six concentrations of OPP, in a static test system for 48 h according to an ASTM Guideline (Standard E729-80). The LC50 value after 48 h was determined to be 2.7 mg a.i./L (nominal) (Dill et al., 1985).

Another test with Daphnia magna and OPP was performed by Kuehn et al. (1988) according to DIN 38412, Part II. However due to some deviations from the guideline and deficiencies found in the report documentation (e.g. purity of test substance is not specified, insufficient description of test conditions, tested a.i. concentrations were not reported) the results should be used as additional information only.