Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To evaluate the acute toxicity of Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)) on Fathead Minnow fish.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : C.I. Direct Blue 218
- Molecular formula : C32H20Cu2N6Na4O16S4
- Molecular weight : 1087.82 g/mol
- Smiles notation : c12c3c(c(S(=O)(=O)[O-])cc1cc(S(=O)(=O)[O-])cc2N)N=Nc1ccc(cc1O[Cu]O3)c1cc2c(N=Nc3c(cc4c(c3O[Cu]O2)c(cc(c4)S(=O)(=O)[O-])N)S(=O)(=O)[O-])cc1.[Na+].[Na+].[Na+].[Na+]
- InChl : 1S/C32H24N6O16S4.2Cu.4Na/c33-19-11-17(55(43,44)45)5-15-9-25(57(49,50)51)29(31(41)27(15)19)37-35-21-3-1-13(7-23(21)39)14-2-4-22(24(40)8-14)36-38-30-26(58(52,53)54)10-16-6-18(56(46,47)48)12-20(34)28(16)32(30)42;;;;;;/h1-12,39-42H,33-34H2,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;;;/q;2*+2;4*+1/p-8/b37-35-,38-36-;;;;;;
- Substance type: Organic
- Physical state: solid powder

Analytical monitoring:

not specified

Details on sampling:

composite stored a t 20 C in dark
Stock solution refrigerated

Vehicle:

not specified

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead Minnow
- Source: Berry water gardens , windmill fish hatcheries , North Carolina
- Length at study initiation : 5.2 cm
- Weight at study initiation: 1.3 g
- Feeding during test: starved 48 hrs before test

ACCLIMATION
- Acclimation period: 10 days
- Acclimation conditions : maintained in plastic stock tank in continuously running charcoal –sand filtered Chapel Hill tap water
- Type and amount of food: protein enriched fish food
- Feeding frequency: 5-7 times per week

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Observations were made after every 24 h

Hardness:

32mg/l

Test temperature:

17°C

pH:

6.8-8.5

Nominal and measured concentrations:

0,18,32,56,100,180 mg/l

Details on test conditions:

Test vessel: 5-gallon wide mouth glass jar
- No. of organisms per vessel: 10

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No other data

Sublethal observations / clinical signs:

Dye concentration mg/l	No. test fish	No. of fish surviving
		24h	48h	72h	96h
0	10	10	10	10	9
18	10	10	10	10	10
32	10	10	10	10	9
56	10	10	10	10	10
100	10	10	10	10	9
180	10	10	10	10	10

Validity criteria fulfilled:

not specified

Conclusions:

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) when subjected to Fathead Minnow (Pimephales promelas) for 96 hrs produced no toxicity and the observed effect concentration was > 180 mg/l.

Executive summary:

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

  10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l

By considering LC50 value it is concluded that test chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria

Description of key information

Short-term toxicity to fish:

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

  10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l

By considering LC50 value it is concluded that test chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

180 mg/L

Additional information

Short-term toxicity to fish:

Experimental study for short-term toxicity to fish was summarized in Handbook of Environment and Waste Management air and water pollution control , Yung-Tse Hung , Lawrence K Wang, Nazih K Shammas 2012 and Ecology committee American Dye Manufacturers Institute, INC. Sept. 1972 for target chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-)) (CAS 28407-37-6).

Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) was used as a test material to evaluate its acute toxicity effect on Fathead Minnow (Pimephales promelas)

  10 Fathead Minnow (Pimephales promelas) were acclimatized in 5-gallon wide mouth glass jar for 10 days before the test in the test temperature 17°C. The test organisms were pre-treated with tetracycline .

  Test material was subjected in the test vessel upto the concentration of 180 mg/l . No toxic effect were observed after 96 hrs. Hence the effect concentration was considered to be >180g/l

By considering LC50 value it is concluded that test chemical Tetrasodium [μ-[[3,3'-[(3,3'-dihydroxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonato]](8-)]]dicuprate(4-) is not toxic tofish and can be considered as “Not classified” according to CLP classification criteria