Tripotassium [5-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-4-hydroxy-3-[(2-hydroxy-4-sulpho-1-naphthyl)azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19.7. – 26.10.2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific principles, acceptable for assessment.

Qualifier:

according to guideline

Guideline:

other: Test No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters."

Deviations:

no

Principles of method if other than guideline:

- Principle of test:
A sample of the tested substance was dissolved in the dilution water at a concentration of 0.1 g/L. The total volume for one dilution is 1000 ml in preliminary test and 4000 ml in limit test.

- Short description of test conditions:
The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.
Test duration 96 hours, the temperature of the solutions 22.0 ± 1.0 °C.

- Parameters analysed / observed:
mortality at 48 and 96 h

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Guppy
- Strain: Poecilia reticulata
- Source: AKVA-SALON, 503 02 Neděliště 51
- Age at study initiation (mean and range, SD): 3 - 4 monts
- Length at study initiation (length definition, mean, range and SD): 2 - 3 cm

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions (same as test or not): same as test

FEEDING DURING TEST
no feeding during test
fasted 72 hours before test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

22.0 ± 1.0 °C

pH:

from 8.26 (0 hour) to 7.78 (96 hour)
control: 7.87 (0 hour) to 7.74 (96 hour)

Dissolved oxygen:

from 8.9 (0 hour) to 8.1 mg/L O2 (96 hour)
control: from 8.9 (0 hour) to 8.2 mg/L

Nominal and measured concentrations:

100 mg.L-1
100.9 mg.L-1

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: open 10 L glass aquarium
- Aeration: without
- No. of organisms per vessel:
Preliminary test: 3 animals
Limit test: 10 animals
- No. of vessels per concentration/control (replicates): no replicates

TEST MEDIUM / WATER PARAMETERS
Dilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled water
A) 117.6 g CaCl3.2H2O in 1L of distilled water
B) 49.3 g MgSO4.7H2O in 1L of distilled water
C) 25.9 g NaHCO3 in 1L of distilled water
D) 2.3 g KCl in 1L of distilled water
The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h

TEST CONCENTRATIONS
- Range finding study: 100, 75, 50, 25 mg.L-1
- Test concentration: 100.9 mg.L-1
- Results used to determine the conditions for the definitive study: no mortality in preliminary test

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Executive summary:

The test substance, Ostazinová modř H-5R , was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 = 100.0 mg.L-1

96 h - LC50 = 100.0 mg.L-1

Description of key information

96 h - LC50 = 100.0 mg.L-1

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L