Nonanoic acid, mixed esters with heptanoic acid, pentaerythritol and valeric acid

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Remarks:

Summary of available data used for the endpoint assessment of the target substance

Adequacy of study:

key study

Justification for type of information:

Refer to analogue justification document provided in IUCLID section 13.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 71010-76-9, Exxon, 2008, respiration inhibition, RL1

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other:

Remarks:

source, CAS 71010-76-9, Exxon, 2008, respiration inhibition, RL1

Duration:

6 h

Dose descriptor:

EC10

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other:

Remarks:

source, CAS 68424-31-7, Croda, 1991, Pseudomonas putida, RL2

Duration:

6 h

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other:

Remarks:

source, CAS 68424-31-7, Croda, 1991, Pseudomonas putida, RL2

Description of key information

No effects up to the limit of water solubility; read-across.

Additional information

No study investigating the toxicity of Monopentaerythritol tetraesters and dipentaerythritol hexaesters of valeric, heptanoic and nonanoic acids to microorganisms is available. Therefore, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read-across to the structurally related source substances Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) and Tetra-esterification products of C5, C7, C8, C10 fatty acids with pentraerythrito (CAS 68424-31-7) is applied.

Based on the high degree of similarity between the structural and physico-chemical properties of the target and source substances, the source substances are considered suitable representatives for the evaluation of the short-term toxicity of the target substance to microorganisms. The read-across approach is justified in detail within the analogue justification in IUCLID section 13.

One study investigating the toxicity of the source substance Decanoic acid, mixed esters with heptanoic acid, octanoic acid, pentaerythritol and valeric acid (CAS 71010-76-9) to aquatic microorganisms is available. The study was conducted under static conditions according to OECD 209 using non-adapted activated sludge obtained from a domestic sewage treatment plant as inoculum. Test concentrations of 10, 100 and 1000 mg/L were prepared. Test substance was added directly to the test vessels. The test substance did not inhibit the respiration rate of activated sludge microorganisms at any of the concentrations employed in the test. Hence, the 3 h-EC50 was determined to be > 1000 mg/L based on the nominal test concentration. Therefore it can be concluded that the test substance will not exhibit effects on the respiration rate of microorganisms.

The study with the source substance Tetra-esterification products of C5, C7, C8, C10 fatty acids with pentraerythrito (CAS 68424-31-7) investigated the toxicity to microorganisms in a cell multiplication inhibition test according to Bringman and Kuehn (1980). Pseudomonas putida was exposed for 6 hours to a static test system at a nominal concentration of 10 mg/L. No inhibition on the growth of Pseudomonas putida was observed at the tested concentration. Hence, the 3 h-EC50 based on growth rate was determined to be > 10 mg/L (EC10 > 10 mg/L) based on the nominal test concentration.

Based on the available results from structurally related source substances (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that Monopentaerythritol tetraesters and dipentaerythritol hexaesters of valeric, heptanoic and nonanoic acids will not exhibit toxicity effects to microorganisms up to the limit of water solubility.