4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of aqutaic algae and cyanobacteria for the test chemical :
1.Short term toxicity of test material to aquatic algae was performed according to the 201 OECD guideline in a static system.
2.To evaluate toxicity of test material on aquatic algae.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid
- Molecular formula: C17H14N2O8S2
- Molecular weight: 438.4356 g/mol
- Smiles notation: c1cc(cc(c1)N)C(=O)Nc2ccc(c3c2c(cc(c3)S(=O)(=O)O)O)S(=O)(=O)O
-InChl:1S/C17H14N2O8S2/c18-10-3-1-2-9(6-10)17(21)19-13-4-5-15(29(25,26)27)12-7-11(28(22,23)24)8-14(20)16(12)13/h1-8,20H,18H2,(H,19,21)(H,22,23,24)(H,25,26,27)
- Substance type: Organic
- physical state: solid

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

1.The stock solution 100 mg/l was prepared by dissolving black powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

Test organisms (species):

other: 1.Desmodesmus subspicatus 2.Green algae

Details on test organisms:

1.TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test temperature:

1) 23±2°C

pH:

1. Test at highest concentration: 7.7 (no change during tests)
Control: 8.0 (changed to 7.5 during test)

Nominal and measured concentrations:

1) 0, 12, 20, 35, 60, 100 mg/l nominal concentrations

Details on test conditions:

1.TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 7.5 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

76.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Read across 1

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

54.3 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Read across 2

Results with reference substance (positive control):

Results with reference substance valid
- EC50: 0.69 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

Toxicity study of test chemical to aquatic algae and cyanobactria was in the concentration range of 54-76 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1. Toxicity study of test chemical to aquatic algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was conducted for 72 hrs. Test was performed according to the 201 OECD guideline in a static system.

The stock solution 100 mg/l was prepared by dissolving black powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the 0, 12, 20, 35, 60, 100 mg/l nominal concentrations. Effects on the growth rate of the organism were studied. Potassium dichromate (K2Cr2O7) were used as a reference positive control. Effects on growth rate were observed for 72 hours by using non linear regression by the software Prism 4.0.

Based on the growth rate inhibition of test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the test material the effect concentration ErC50 was 76.8 mg/l.

2.Test material was used to evaluate its toxicity on aquatic green algae .The effect concentration of test chemical on green algae after 96 h was observed to be 54.3 mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 54 -78 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for toxicity for aquatic algae and cynobacteria as "Aquatic tox 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the dose range of 54 -78 mg/L .

Description of key information

Toxicity to aquatic algae and cynobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.

Short term toxicity study of test chemical to aquatic algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was conducted for 72 hrs. Test was performed according to the 201 OECD guideline in a static system.

The stock solution 100 mg/l was prepared by dissolving black powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the 0, 12, 20, 35, 60, 100 mg/l nominal concentrations. Effects on the growth rate of the organism were studied. Potassium dichromate (K2Cr2O7) were used as a reference positive control. Effects on growth rate were observed for 72 hours by using non linear regression by the software Prism 4.0.

Based on the growth rate inhibition of test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the test material the effect concentration ErC50 was 76.8 mg/l.

2.Test material was used to evaluate its toxicity on aquatic green algae .The effect concentration of test chemical on green algae after 96 h was observed to be 54.3 mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 54 -78 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for toxicity for aquatic algae and cynobacteria as "Aquatic tox 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the dose range of 54 -78 mg/L .

Key value for chemical safety assessment

EC50 for freshwater algae:

78 mg/L

Additional information

Toxicity to aquatic algae and cynobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid (70239 -77 -9).The studies are as mentioned below:

1.

Short term toxicity study of test chemical to aquatic algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was conducted for 72 hrs. Test was performed according to the 201 OECD guideline in a static system.

The stock solution 100 mg/l was prepared by dissolving black powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the 0, 12, 20, 35, 60, 100 mg/l nominal concentrations. Effects on the growth rate of the organism were studied. Potassium dichromate (K2Cr2O7) were used as a reference positive control. Effects on growth rate were observed for 72 hours by using non linear regression by the software Prism 4.0.

Based on the growth rate inhibition of test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the test material the effect concentration ErC50 was 76.8 mg/l.

2.Test material was used to evaluate its toxicity on aquatic green algae .The effect concentration of test chemical on green algae after 96 h was observed to be 54.3 mg/l.

Thus, based on the above summarised studies, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range of 54 -78 mg/ L. Thus, comparing this value with the criteria of CLP regulation, 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid can be classified for toxicity for aquatic algae and cynobacteria as "Aquatic tox 3" .Hence,based on the data available for the structurally and functionally similar read across, test chemical 4-[(3-aminobenzoyl)amino]-5-hydroxynaphthalene-1,7-disulphonic acid is likely to be toxic atleast in the dose range of 54 -78 mg/L .