Ammonium iodide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard OECD test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test chemical solution was prepared by dissolving 2 mg of the test chemical in 20 ml of ADaM’s media achieving test concentrations of 100 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Food: No feeding after hatching

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

153.5 mg of CaCO3

Test temperature:

20±2°C

pH:

7.1

Nominal and measured concentrations:

Nominal concentrations: 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Immobility

Nominal concentrations (mg/l)	Number of Daphnids	Immobility			Total live daphnids after 48 hr of exposure period
		0 hr	24 hr	48 hr
Control	10	-	-	-	10
100	10	-	5	-	5

 

pH and oxygen content:

Nominal Concentration (mg/l)	Number of Daphnids	pH(mg/L)
		0 h	48h
Control	10	7.2	7.4
100	10	7.3	7.5

 

Nominal Concentration (mg/l)	Number of Daphnids	DO(mg/L)
		0 h	48h
Control	10	7.1	7.0
100	10	7.0	6.9

 

Test temperature:

Nominal Concentration (mg/l)	Number of Daphnids	Temparature
		0 h	48h
Control	10	21.2	21.3
100	10	21.2	21.3

 

Validity criteria fulfilled:

yes

Remarks:

1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels

Conclusions:

Based on nominal concentrations, experimental median effective concentrations [EC50 (48 h)] for test chemical on Daphnia magna was determined to be 100 mg/L.

Executive summary:

An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test chemical solution was prepared by dissolving 2 mg of the test chemical in 20 ml of ADaM’s media achieving test concentrations of 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical solution. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, pH 7.1, hardness of water 153.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. The daphnids were moving slowly as compared to control. No immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be 100 mg/L.

Description of key information

An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test chemical solution was prepared by dissolving 2 mg of the test chemical in 20 ml of ADaM’s media achieving test concentrations of 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical solution. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, pH 7.1, hardness of water 153.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. The daphnids were moving slowly as compared to control. No immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202“Daphnia sp.,Acute Immobilization Test”. The test chemical solution was prepared by dissolving 2 mg of the test chemical in 20 ml of ADaM’s media achieving test concentrations of 100 mg/L. Thus, limit test was performed using 100 mg/l of test chemical solution. Study was performed using 10 daphnids in a static system. Total 10 Daphnids/conc. were exposed to test chemical in 25 ml beakers in a volume of 20 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±2°C, pH 7.1, hardness of water 153.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. One control vessel was also run simultaneously during the study. The animals were exposed to medium (i.e.a beaker containing only medium) and the test chemical concentrations for a period of 48 hour. The daphnids were moving slowly as compared to control. No immobility were found in the control test animals and the dissolved oxygen concentration at the end of the test in the control and test vessel was ≥ 3 mg/l, thus validity criterion of the study has been fulfilled. On the basis of effect of test chemical on mobility of the test organism, the median effect concentration (EC50 (48 h)) value was determined to be 100 mg/L.