1,2-dihydroxyanthraquinone

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian cell study: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Evaluation of DNA-binding activity of hydroxyanthraquinones occuring in Rubia tinctorum L

Author:

B. Poginsky et al.

Year:

1991

Bibliographic source:

Carcinogenesis vol. 12 no. 7, pp. 1265 - 1271

Report date:

1991

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Principle of test: In the present study they have investigated by 32P-postlabelling the possible binding to DNA of test substance in vivo, following oral administration of the test substance in mice.

GLP compliance:

no

Type of assay:

other: DNA-binding activity: 32P-postlabelling assay (detection of covalent binding of chemical carcinogens to DNA in animals and humans)

Test material

Test material information

Constituent 1

Reference substance name:

1,2-dihydroxyanthraquinone

EC Number:

200-782-5

EC Name:

1,2-dihydroxyanthraquinone

Cas Number:

72-48-0

Molecular formula:

C14H8O4

IUPAC Name:

1,2-dihydroxy-9,10-dihydroanthracene-9,10-dione

Test material form:

solid: particulate/powder

Test animals

Species:

mouse

Strain:

other: Parkers

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: National Institute for Medical Research, Mill Hill, London
- Age at study initiation: 4-6 weeks
- Weight at study initiation: n.a.
- Fasting period before study: deprived of food each evening, the compounds was administered the following morning
- Housing: n.a.
- Diet (e.g. ad libitum): ad libitum for the rest of the day
- Water (e.g. ad libitum): ad libitum
- Acclimation period: n.a.

Administration / exposure

Route of administration:

oral: feed

Vehicle:

- Concentration of test material : 10mg

Details on exposure:

DIET PREPARATION
- Rate of preparation of diet (frequency):1/day
- Mixing appropriate amounts with (Type of food): cheese spread (1g)
- Storage temperature of food: n.a.

Duration of treatment / exposure:

4 days

Frequency of treatment:

1/day

Post exposure period:

4 hours after the last administration the mice were killed

Doses / concentrations

Dose / conc.:

10 other: mg/day

No. of animals per sex per dose:

2 animals per group, one dose

Control animals:

yes, concurrent vehicle

Positive control(s):

none

Examinations

Tissues and cell types examined:

Liver, kidney, duodenum and colon were removed and stored at -70°C until DNA isolation

Results and discussion

Test results

Key result

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

valid

Additional information on results:

The 32P-postlabelling analysis of hepatic DNA from mice treated with the test item does not show evidence of radioactive spot other than those seen in control DNA.
The kidney DNA from mice treated with the test substance showed a similar pattern to that of the control group.

Applicant's summary and conclusion

Conclusions:

The test substance does not form DNA adducts in mice liver, kidney, duodenum and colon.

Executive summary:

In the present study, they have investigated by³²P-postlabelling the possible binding to DNA of 1,2 -dihydroantraquinone in vivo, following the treatment of males Parkes mice, 4 -6 weeks old. The³²P-postlabelling assay is a method to detect covalent binding of chemical carcinogens to DNA in animals and humans.

Duplicate group of two animals/group were deprived of food each evening and the compounds (10mg/d) were administered mixed with cheese spread (1g) the following morning. Thereafter, the mice received standard lab diet ad libitum for the rest of the day. The mice were treated for 4 days; the controls received cheese spread only. Four hours after the last administration, the mice were killed. Liver, kidney, duodenum and colon were removed and store at -70°C until DNA isolation.

The thawed tissues sample were homogenized and the DNA were isolated and labelled by³²-P-postlabelling. The DNA adducts were measured by chromatography. The presence of radiolabelled adducts on the chromatograms was detected by autoradiography. The levels of adducts in DNA samples were determined from the amount of radioactivity present in the adduct spot, measured by Cerenkov counting³²P-ATP used in the labelling reaction.

In conclusion, the³²P-postlabelling of DNA from mice treated with the test substance did not give rise of DNA adducts in mice liver, kidney, duodenum and colon.