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Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

other: aerobic sludge

Details on inoculum:

aerobic sludge selected by the mied treatment plant of urban (about 66%) and industrial (about 34%) liquid sewage situated at San Rocco-Monza.

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

other: organic carbon (TOC)

Parameter followed for biodegradation estimation:

inorg. C analysis

Details on study design:

15 bottles have been prepared as follows:
- 5 bottles for the test item
- 5 bottles for the blank
- 5 bottles with the reference substance (sodium benzoate)
to which the inoculum of the muds has been added.

Reference substance:

other: sodium benzoate

Preliminary study:

None

Test performance:

Quality criteria of the test are satisfied.
The percentage of average degradation of the reference substance is 114% (> 60%) after 14 days of incubation.

Parameter:

% degradation (inorg. C analysis)

Value:

ca. 114

Sampling time:

14 d

Parameter:

% degradation (inorg. C analysis)

Value:

ca. 122

Sampling time:

28 d

Details on results:

Test item (Kerastim) :
% of biodegradation after 1 day of incubation = 23
% of biodegradation after 7 days of incubation = 29
% of biodegradation after 14 days of incubation = 114
% of biodegradation after 20 days of incubation = 101
% of biodegradation after 28 days of incubation = 122

Results with reference substance:

Reference substance (sodium benzoate) :
% of biodegradation after 14 days of incubation = 111
% of biodegradation after 28 days of incubation = 115

Validity criteria fulfilled:

yes

Remarks:

the percentage of average degradation of the reference substance is > 60% after 14 days of incubation.

Interpretation of results:

readily biodegradable

Conclusions:

On the base of results obtained, interpreted in accordance to OECD 301:1992, OECD Section 3 Part 1: 2006 the test item "KERASTIM should be considered biodegradable in aerobic condition.

Executive summary:

On the test item, KERASTIM S, the analysis of inorganic carbon for the evaluation in an aqueous medium of the aerobic biodegradability has been performed following screening method described in OECD 301:1992.

For this purpose the amount of inorganic carbon developed has been reported in comparison to the blank.

The test has been done using the test item with a concentration of about 20 mg/l of organic carbon. Samples have been kept at the temperature of 25°C for the whole period of the test (28 days).

On the base of results obtained, interpreted in accordance to OECD 301:1992, OECD Section 3 Part 1: 2006 the test item "KERASTIM S" should be considered biodegradable in aerobic condition.

Description of key information

On the base of results obtained, interpreted in accordance to OECD 301:1992, OECD Section 3 Part 1: 2006 the test item "KERASTIM S" should be considered biodegradable in aerobic condition.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable