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EC number: 246-618-6 | CAS number: 25103-54-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 August 2017- 22 September 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Remarks:
- Toxicity control conducted as part of an OECD 301B study
- Qualifier:
- according to guideline
- Guideline:
- other: Toxicity control conducted as part of an OECD Guideline 301B, Section 3. Degradation and Accumulation. "Ready Biodegradability: CO2 Evolution Test"
- Version / remarks:
- 17 July, 1992
- Deviations:
- no
- Principles of method if other than guideline:
- Result taken from a toxicity control conducted as part of an OECD 301B study.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Toxicity control: Test item and sodium acetate (reference item)
- Method: On the day of testing weighed amounts of the test item were added to the 2-litres test bottles containing medium with microbial organisms and mineral components (toxicity control bottle: 44.2 mg). To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test, to ensure optimal contact between the test item and the test organisms. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge: An activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: Before use the sludge was allowed to settle (33 minutes) and the supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium.
- Concentration of suspended solids: 4.2 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 22.2 - 23.2°C
- pH:
- Test start: 7.6
Test end: 8.0 - Nominal and measured concentrations:
- Target test item concentration: 22 mg/L (12 mg TOC/L)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 2 litre brown coloured glass bottle
- Aeration: Test media were aerated and stirred continuously
- No. of vessels per concentration (replicates): 1
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: Excluded from light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Inhibition of microorganisms leading to decreased CO2 production
TEST CONCENTRATIONS
- Range finding study : Not applicable
- Test concentrations: 22 mg/L - Reference substance (positive control):
- yes
- Remarks:
- sodium acetate
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition of microorganisms leading to decreased CO2 production in a toxicity control conducted as part of a ready biodegradation study
- Remarks on result:
- other: Toxicity control in an OECD 301B study did not demonstrate the test item is toxic to microorganisms
- Details on results:
- In the toxicity control, more than 25% biodegradation occurred within 14 days (36%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity.
- Results with reference substance (positive control):
- In the same biodegradation study, the positive control demonstrated 79% degradation after 14 days, therefore meeting the validity criteria,
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity control in an OECD 301B study demonstrated that the test item is not toxic to microorganisms at 22 mg/L. This is therefore considered to be the NOEC for toxicity to STP microorganisms.
- Executive summary:
Toxicity to microorganisms was assessed based on the toxicity control included as part of an OECD 301B ready biodegradation study (Timmer 2017). The toxicity control consisted of test item (22 mg/L) and reference item (sodium acetate). As 36% degradation was demonstrated after 14 days for the toxicity control it was concluded that the test item is not inhibitory to microorganisms at this concentration. The NOEC for toxicity to STP microorganisms is therefore taken as 22 mg/L.
Reference
Table 1 CO2Production and Percentage Biodegradation of the Toxicity Control
Day |
HCl(0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
|
Blank (mean) |
Toxicity control |
|||||
1 |
48.23 |
47.94 |
0.29 |
0.3 |
0.3 |
0 |
4 |
46.49 |
26.83 |
19.66 |
21.6 |
21.9 |
13 |
6 |
46.30 |
36.69 |
9.61 |
10.6 |
32.5 |
19 |
8 |
46.03 |
40.91 |
5.12 |
5.6 |
38.1 |
22 |
11 |
46.92 |
40.30 |
6.62 |
7.3 |
45.4 |
26 |
152) |
45.89 |
29.41 |
16.48 |
18.1 |
63.6 |
36 |
1): Calculated as the ratio between CO2produced (cumulative) and the sum of the ThCO2of the test item and positive control: 174.5 mg CO2/2L (ThCO2test item: 88.8 mg CO2/2L + ThCO2sodium acetate: 85.7 mg CO2/2L). 2): CO2measured on day 15 is actually part of CO2production of day 14, since microbial activity was ended on day 14 by addition of HCl. |
Description of key information
The toxicity control in an OECD 301B study demonstrated that the test item was not toxic to microorganisms at 22 mg/L. This is therefore considered to be the NOEC for toxicity to STP microorganisms.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 22 mg/L
Additional information
Toxicity to microorganisms was assessed based on the toxicity control included as part of an OECD 301B ready biodegradation study (Timmer 2017). The toxicity control consisted of test item (22 mg/L) and reference item (sodium acetate). As 36% degradation was demonstrated after 14 days for the toxicity control it was concluded that the test item is not inhibitory to microorganisms at this concentration. The NOEC for toxicity to STP microorganisms is therefore taken as 22 mg/L.
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