Zinc bis(N-ethyl-N-phenyldithiocarbamate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

UBA test protocol, Mai 1984 (Umweltbundesamt Berlin)

Deviations:

not specified

GLP compliance:

no

Specific details on test material used for the study:

Purity: 100%

Analytical monitoring:

no

Remarks:

Due to limited solubility of the test substance (0.07 mg/L), control analysis were not carried out.

Vehicle:

yes

Remarks:

Ultra-Turrax

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Type: Brachydanio rerio (Hamilton Buchanan)
Date of birth: 05.04.1990
Date of delivery: 27.06.1990
Body length: 2.5 - 3.5 cm
Mortality during acclimatisation period: no
Medical pre-treatment: no
Keeping water: synthetic fresh-water acc. to ISO

Test type:

static

Water media type:

freshwater

Remarks:

synthetic

Total exposure duration:

96 h

Test temperature:

21.0 - 22.1 °C

pH:

7.0 - 8.0

Dissolved oxygen:

7.8 - 8.6 mg/L

Nominal and measured concentrations:

test concentrations: 3.16, 10, 31.6, 100, 316, 1000 mg/L

Details on test conditions:

Fish tank: 300 x 135 x 200 mm
5L test medium
aired

Reference substance (positive control):

yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

562 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Test substance was added to water and treated with Ultra-Turrax for 60 Seconds. In case of all test concentrations unsolved substance in powder form could be seen on surface of test medium.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

316 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Test substance was added to water and treated with Ultra-Turrax for 60 Seconds. In case of all test concentrations unsolved substance in powder form could be seen on surface of test medium.

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Test substance was added to water and treated with Ultra-Turrax for 60 Seconds. In case of all test concentrations unsolved substance in powder form could be seen on surface of test medium.

Details on results:

The LC 0 and LC 100 values were determined directly. The LC 50 value was determined computationally and graphically applying the dose-response curve. The test duration is 96h.

Sublethal observations / clinical signs:

Result of control: no mortality.

In case of all nominal test concentrations (3.16, 10, 31.6, 100, 316 and 1000 mg/L) a normal swimming behaviour of fishes could be observed.

Validity criteria fulfilled:

yes

Remarks:

Inspection of test, raw data and report were controlled by the quality assurance at times.

Conclusions:

Vulkacit P Extra N was tested on short-term toxicity to fish and a LC 50 value of 562 mg/L was determined.

Executive summary:

Vulkacit P Extra N was tested on short-term toxicity to fish. A LC 50 value of 562 mg/L was determined via dose-response curve using measured values to LC 0 (316 mg/L) and LC 100 (1000 mg/L). Analysis was performed on Danio rerio under static and GLP conditions according to UBA test protocol (comparable to OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test').

Description of key information

Vulkacit P Extra N was tested on short-term toxicity to fish. A LC 50 value of 562 mg/L was determined via dose-response curve using measured values to LC 0 (316 mg/L) and LC 100 (1000 mg/L). Analysis was performed on Danio rerio under static and GLP conditions according to UBA test protocol (comparable to OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test').

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

562 mg/L