2-methyl-1,4-phenylene-bis[4-(6-acryloyloxyhexyloxy)benzoate]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 12 - May 14, 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

July 1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Remarks:

The test material concentrations in the study were under the limit of quantification and no analysis were performed.

Vehicle:

yes

Remarks:

reconstituted water

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test medium (reconstituted water and test material) was freshly prepared. Therefore, the calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated, and stirred with a magnetic stirrer for further 23 hours. After 24 hours the formulation was given through a nutsch filter (pore size >10 - <16 μm). The filtrate was used for the study.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna STRAUS
- Source: Merck KGaA, Darmstadt Institute of Toxicology
- Age of parental stock (mean and range, SD): not older than 24 hours
- Feeding during test: none

ACCLIMATION
- Acclimation conditions: same as test
- Type and amount of food:suspension of fresh water algae
- Feeding frequency: once a week
- Health during acclimation (any mortality observed):

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: The strain with the parent generation was bred and maintained in vessels containing a batch of Daphnia magna of different ages. Preparing a new study, juvenile Daphnia magna were separated and placed in 100 mL of reconstituted water. The water was renewed and the Daphnia magna were fed with a suspension of fresh water algae once a week. Newborn animals were separated and allocated to the different dose groups.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg/L expressed as CaCO3

Test temperature:

21 to 22°C

pH:

7.74 - 8.19

Dissolved oxygen:

97.5 - 99.4%

Salinity:

NA

Nominal and measured concentrations:

nominal: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels
- Type: open
- Material, size, fill volume: glass, 25 mL, 10 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to ELENDT M4 (1990). After preparation the reconstituted water was aerated for 24 hours. The hardness: 250 mg/L expressed as CaCO3 and the pH: 7.9 ± 0.3 after an aeration for
24 hours.
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light/ 8 hours darkness

EFFECT PARAMETERS MEASURED: mobility after 24 and 48 hours

VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

no

Remarks:

No positive control used in this study. The accuracy and reliability of the test method is demonstrated periodically as recommended by guidelines with potassium dichromate.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: EC50 > 0.0378 mg/L

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: EC50 > 0.0378 mg/L

Details on results:

- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 for the test material to Daphnia magna could not be quantified due to the absence of toxicity at the highest test concentration achievable in water. An aqueous solution of 100 mg/L revealed no aquatic toxicity in the test system.

Executive summary:

The objective of this study was to determine the acute toxicity of the test material in a static test to Daphnia magna.

For this purpose, juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours, under defined conditions.  Daphnia magna were exposed to an aqueous solution from a test material concentration of nominal 100 mg/L (limit-test) in an open system. Daphnia magna exposed to an aqueous solution of the nominal concentration of 100 mg/L were not affected.

The analysis of a saturated aqueous solution revealed that the water solubility of the test item was < 0.0000378 g/L. The limit of quantification of the analytical method was described to be about 0.000126 g/L. Therefore, the test material concentrations in the aqueous medium at the start and the end of the study could not be quantified.

In conclusion, the 48 hour EC50 for the test material to Daphnia magna could not be quantified due to the absence of toxicity at the highest test concentration achievable in water. An aqueous solution of 100 mg/L revealed no aquatic toxicity in the test system.

Description of key information

The 48 h EC50 could not be quantified due to the absence of toxicity at the highest concentration achievable in water. Thus, the EC50 value is greater than 100 mg/L.

Additional information

The objective of this study was to determine the acute toxicity of the test material in a static test to Daphnia magna according to OECD Guideline 202.

Daphnia magna were exposed to an aqueous solution from a test material concentration of nominal 100 mg/L (limit-test) in an open system for 48 hours. Daphnia magna exposed to an aqueous solution of the nominal concentration of 100 mg/L were not affected.

The analysis of a saturated aqueous solution revealed that the water solubility of the test item was < 0.0000378 g/L. The limit of quantification of the analytical method was described to be about 0.000126 g/L. Therefore, the test material concentrations in the aqueous medium at the start and the end of the study could not be quantified.

The 48 hour EC50 for the test material to Daphnia magna could not be quantified due to the absence of toxicity at the highest test concentration achievable in water. An aqueous solution of 100 mg/L revealed no aquatic toxicity in the test system.