Triphenylphosphine oxide

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Principles of method if other than guideline:

Skin irritation was tested using an internal standard method (BASF test). White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and two other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid or powdered and moistened test substance.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine oxide

EC Number:

212-338-8

EC Name:

Triphenylphosphine oxide

Cas Number:

791-28-6

Molecular formula:

C18H15OP

IUPAC Name:

(diphenylphosphoroso)benzene

Test material form:

solid

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: 2.66 -3.09 kg

ENVIRONMENTAL CONDITIONS
not reported

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

water

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount applied: ca. 1 mL
- Concentration: 80 %

Duration of treatment / exposure:

Experiment 1: 1, 5, or 15 minutes
Experiment 2: 20 hrs

Observation period:

Experiment 1: up to 8 days
Experiment 2: up to 8 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: An application site of 2.5 X 2.5 cm was covered with the liquid (37 °C; approx. 1 mL)

REMOVAL OF TEST SUBSTANCE
- Washing:
Experiment 1: washing was done in the 1 min, 5 min and 15 min test directly after exposure. Lutrol 50 % (mild detergent) was used.
Experiment 2: No washing was done after the 20 h treatment.

Descriptive scores of the raw data have been converted to Draize numerical scores.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Remarks:

20 h exposure

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: no effects

Results 2

Irritation parameter:

edema score

Remarks:

20 h exposure

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: no effects

Irritant / corrosive response data:

No effects were also observed in the experiment with short term exposures (1, 5 and 15 min)

Applicant's summary and conclusion

Interpretation of results:

not irritating

Conclusions:

In a study according to an internal method, the test item was determined to be not irritant to skin of rabbit.

Executive summary:

The skin irritation of the test item was assessed using an internal standard method (BASF test). White Vienna rabbits (weight at study initiation: 2.66 -3.09 kg) were used. Two animals were treated for 1, 5 and 15 minutes and two other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the powdered and moistened test substance. An application site of 2.5 X 2.5 cm was covered (37 °C; approx. 1 mL of an 80 % test item solution; vehicle = water). The observation period was up to 8 days. In result, no effects were observed. Both the mean erythema score (20 h) and the mean edema score (20 h) was 0 (max score = 4). No effects were also observed in the experiment with short term exposures (1, 5 and 15 min). In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item is not irritant to skin.