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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-01-12 (date of study plan) - 1996-03-01 (date of final report)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Remarks:
- DIN-Norm 38 412 L8, GLP
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Version / remarks:
- DIN-Norm 38 412 L8
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Remarks:
- aqua ad iniectabilia
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: WAF
- Eluate: water
- Differential loading: 10 g/l - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture:
Stock culture was sored frozen at -80°C in Casein-Soyflourpepton-boullon with 7.5 % DMSO
- Method of cultivation: Working culture was stored in solid medium in agar tubes. New cultures were prepared every week and cultured at 25°C
- Preparation of inoculum for exposure:
Pre-culture: The inoculum for the pre-culture was taken from a up to 7 day old stem culture under sterile conditions to reveal a turbidity of approx. TE/F = 10 in the pre-culture. the preparation was incubated for 7 hours at 21±1°C. Afterwards it was diluted to reveal a defined turbidity of approx. TE/F = 50.
Test culture: The diluted preparations (each 80 ml) of the test item were added into test vessels (300 ml Erlenmeyer flasks) with 10 ml test culture solution and 10 ml of the TE/F = 50 inoculum to reveal a calculated turbidity of TE/F = 5. Afterwards the test cultures were incubated in triplicates at 21±1°C for 16±1 hour. As control, 5 Erlenmeyer flasks with 80 ml water instead of the test item solution were incubated, too.
- Source: Deutsche Sammlung von Mikroorganismen (Braunschweig, Germany) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Test temperature:
- 21±1°C
- Nominal and measured concentrations:
- Nominal: 3.0, 1.5, 0.75, 0.38, 0.19, 0.1, 0.05, 0.025, 0.013, 0.007, 0.0035 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 ml Erlenmeyer flasks
- Material, size, headspace, fill volume: 100 ml fill volume
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : turbidity
TEST CONCENTRATIONS
- Spacing factor for test concentrations: factor 2 - Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.004 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr. (dissolved fraction)
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The toxicity of the test item towards microorganisms was determined scientifically reasonable acc. DIN-Norm 38 412 L8, the given information indicates that testing was properly conducted, hence, the results can be considered sufficiently reliable. The EC10 was determined as 0.0035 mg/l, the EC50 to 0.4 mg/l.
- Executive summary:
The toxicity of the test item towards Pseudomonas putida was determined scientifically reasonable acc. DIN-Norm 38 412 L8. For the main experiment two stock solutions of the test item, 5g ad 500 ml aqua ad iniectabilia, were prepared and stirred with a magnetic stirrer for 24h at room temperature. The highest concentration was prepared by 1:2,5 dilution of the pooled and clear filtered stock solution. Measured concentration of the stock solution was 3.7 mg/l, so it was tested at the following concentrations: 3.0, 1.5, 0.75, 0.38, 0.19, 0.1, 0.05, 0.025, 0.013, 0.007, 0.0035 mg/l. pH was not adjusted. Test item was tested in triplicates, control in 5 replicates. Effective concentrations were determined by graphical plot of the measured values of the mean experiment. The EC10 was determined as 0.0035 mg/l, the EC50 to 0.4 mg/l.
Reference
Description of key information
Toxicity to microorganisms: EC50(16h) = 0.4 mg/l for Pseudomonas putida (static, DIN-Norm 38 412 L8, GLP)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 0.4 mg/L
- EC10 or NOEC for microorganisms:
- 0.004 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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