Reaction products of tetrabromophthalic anhydride with 2,2'-oxydiethanol and methyloxirane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed prior to international guidelines. Performed according to GLPs. Used 5 test concentrations and negative/positive controls.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Committee on methods for toxicity tests with aquatic organisms. 1975. Methods for acute toxicity tests with fish, macroinvertebrates adn amphibians. EPA, Ecological Research Series EPA-660/3-75-009, April, 1975. 61 pp.

Principles of method if other than guideline:

Static test. 5 test concentraitons plus negative and positive controls. Observations at 24, 48, 96 hr post addition of test material to tanks.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-hydroxyethoxy)ethyl 2-hydroxypropyl 3,4,5,6-tetrabromophthalate

EC Number:

243-885-0

EC Name:

2-(2-hydroxyethoxy)ethyl 2-hydroxypropyl 3,4,5,6-tetrabromophthalate

Cas Number:

20566-35-2

Molecular formula:

C15H15Br4O7 (one ring component) C8 H2 Br4 O4 . C4 H10 O3 . C3 H8 O2 Unspecified reaction product of the three components

IUPAC Name:

2-(2-hydroxyethoxy)ethyl 2-hydroxypropyl 3,4,5,6-tetrabromophthalate

Details on test material:

FM PHT-4 Diol commercial product.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

See below.

Test organisms

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

See below.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

45 mg/L CaCO3

Test temperature:

22 +/- 1 degrees C

pH:

7.0

Dissolved oxygen:

9.4 mg/L

Nominal and measured concentrations:

FM PHT-4 Diol: 0, 10, 18, 32, 56 and 100 mg/L.
Antimycin A: positive control

Reference substance (positive control):

yes

Remarks:

Antimycin A

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: The 95% CI was 0-18 mg/L.

Details on results:

At the 32, 56 and 100 mg/L concentrations, 100% mortality was observed at 24 hr. In the 18 mg/L concentration, 90% and 100% mortality were observed at 24 and 48 hrs, respectively. At the 10 mg/L concentration, no mortality was observed at 24 and 48 hr and 20% mortality was observed at 96 hrs. No mortality occurred in the negative control group.

Results with reference substance (positive control):

Performed as expected. 96 hrLC50=0.000056 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

An acute static fish toxicity study was conducte with FM PHT-4 Diol. Bluegill sunfish were exposed to concentrations of 10, 18, 32, 56 and 100 mg test substance/L water. Acetone was used as vehicle. All test concentrations were cloudy with the 56 and 100 mg/L levels being completely opaque. The 96-hr LC50 was 12 mg/L with 95% confidence limits of 0-18 mg/L. A 96 -hr no observed effect concentration of <10 mg/L was indicated.

Executive summary:

An acute static fish toxicity study was conducte with FM PHT-4 Diol. Bluegill sunfish were exposed to concentrations of 10, 18, 32, 56 and 100 mg test substance/L water. Acetone was used as vehicle. All test concentrations were cloudy with the 56 and 100 mg/L levels being completely opaque. The 96-hr LC50 was 12 mg/L with 95% confidence limits of 0-18 mg/L. A 96 -hr no observed effect concentration of <10 mg/L was indicated.