Zinc dodecyl hydrogen disulphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 - 24 Nov 2014

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
The test solution was prepared in the concentration 2.1 g/L in water. Five concentrations were preped by dilutions in water.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: not stated
- Length at study initiation: 2 +/- 1 cm
- Weight at study initiation: not stated
- Method of breeding: not stated
- Feeding during test: none

ACCLIMATION
- Acclimation period: 19 days (range finding test), 14 days (definitive test)
- Acclimation conditions (same as test or not): same
- Type and amount of food: feedstuff in flakes
- Feeding frequency: 3 times a week
- Health during acclimation (any mortality observed): no mortality was observed

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

240 mg CaCO3/L

Test temperature:

22.8 - 23.6°C

pH:

Control: 7.53 - at test initiation; 8.12 at test end
Test samples: 7.54 at test initiations; 8.41 at test end

Dissolved oxygen:

Control: 71.8% - at test initiation; 67.8% at test end
Test samples: 67.6% at test initiations; 66.6% at test end

Nominal and measured concentrations:

0; 14.50; 17.4; 20.89; 25.06; 30.08 mg/L (nominal for tested product)

Details on test conditions:

TEST SYSTEM
- Test vessel: 60L glass tanks
- Type (delete if not applicable): open / closed
- Aeration: continuous with porous stones
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 1
- Biomass loading rate: less than 1 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
the water used for assay was allowed to stabilize for 48 hours prior the test initiation.

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12h light/ 12h dark
- Light intensity: not stated

EFFECT PARAMETERS MEASURED: mortality and visible abnormalities at 2, 4, 24, 48, 72, 96 hour

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.56 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Other adverse effects control: none

Sublethal observations / clinical signs:

Table 1. Mortality results - definitive test:

Test concentration [mg/L]	Time
	24h	48h	72h	96h
0	0/7	0/7	0/7	0/7
14.5	0/7	0/7	1/7	1/7
17.4	2/7	4/7	4/7	4/7
20.89	6/7	6/7	6/7	6/7
25.06	7/7	7/7	7/7	7/7
30.08	7/7	7/7	7/7	7/7

Description of key information

96h LC50 = 4.56 mg a.i./L (Brachydanio rerio, nominal concentration,  OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.56 mg/L

Additional information

One study on short-term toxicity to fish is available for C12-14 AS Zn. The GLP, static test was performed according to OECD 203 guideline (Z&S, 2014). Zebrafish (Brachydanio rerio) were exposed for 96-hour to several test concentrations: 0; 14.50; 17.40; 20.89; 25.06; 30.08 mg product/L (nominal values). The tested product had a purity of 26.6% and the remaining was water. The TOC analysis proved that the test concentrations are stable over time. Since no other analytical measurements of the test substance were performed, the results are based on the nominal concentrations. The 96-hour LC50 value was 17.13 mg product/L and 4.56 mg a.i./L (nominal value).