[No public or meaningful name is available]

Additional information on environmental fate and behaviour

Administrative data

Endpoint:

additional information on environmental fate and behaviour

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study following sound scientific principles. This is a non-standard, proprietary study specifically designed and conducted in order to provide supporting evidence to the testing program. Acceptable with restrictions.

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

no guideline followed

Deviations:

not applicable

Principles of method if other than guideline:

Ecotoxicity screening studies were conducted in which a biomimetic extraction technique using solid-phase microextraction (SPME) in conjunction with gas chromatography (GC) was employed to assess the level of water soluble hydrocarbons and hence the potential toxicity of the water accommodated fractions (WAFs) of the test samples. In addition, the toxicity of WAFs was also monitored using an in-vitro MicrotoxTM assay, a biosensor-based measurement system.

GLP compliance:

no

Type of study / information:

Leaching of the thickener from base oils for a range of greases

Test material

Test material information

Constituent 1

Reference substance name:

Tetraurea 2

IUPAC Name:

Tetraurea 2

Test material form:

other: Grease: thickener in a base oil

Details on test material:

Tetraurea 2 base grease in normal base oil

Results and discussion

Any other information on results incl. tables

Results and Conclusions

The SPME and Microtox results for the WAFs of the grease samples at 1000 mg/L loading rate are as follows:

Grease sample	SPME-GC	Microtox reading
	(Total peak area)	(5 min)	(15 min)
Tetraurea thickener 2 base grease	503,192	>100	>100

Both sets of screening data confirm that there is no apparent toxicity associated with any of the samples at the 1000 mg/L loading rate. The SPME-GC data summarised above, supported by the chromatograms, confirm that the total peak area counts are all extremely low and equivalent to background readings associated with a control sample (i.e. a WAF without any test sample added).

The screening ecotoxicity data (using SPME-GC and the in-vitro Microtox assay), confirm a lack of toxicity of the greases.

Applicant's summary and conclusion

Conclusions:

There is negligible leaching of the thickeners from base oils in the samples tested. The SPME-GC data confirm that the total peak area counts are all extremely low and equivalent to background readings associated with a control sample.

Executive summary:

It was proposed that leaching studies be undertaken on thickeners in base oils to provide additional support to the grease thickener testing strategy. Information generated during the screening studies will be of relevance and value to the risk assessment of a wide range of grease thickeners and to assess the potential bioavailability of grease and other lubricant components.

The approach for the PU thickeners was to use a solid-phase microextraction (SPME) method combined with gas chromatography (GC) to assess the bioaccumulation potential of the water accommodated fractions (WAFs) of PU based grease. This approach was also used to assess the availability of the organic complexes of the metal based grease thickeners (e.g. Lithium based complexes) and complemented with metal ion analysis.

Ecotoxicity screening studies were conducted in order to determine whether there was any toxicity associated with the grease samples. The toxicity of WAFs was monitored using an in-vitro Microtox assay, a biosensor-based measurement system. The screening ecotoxicity data (using SPME-GC and the in-vitro Microtox assay), confirm a lack of toxicity of the greases.