Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- assessment report
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Remarks:
- pH 6.8, DOC 17.3 mg/L, Ca 38.3 mg/L
- Effect conc.:
- 3.25 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Remarks:
- pH 7.3, DOC 2.53 mg/L, Ca 5 mg/L
- Effect conc.:
- 0.68 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Remarks:
- pH 6.0, DOC 5.37 mg/L, Ca 3.7 mg/L
- Effect conc.:
- 0.38 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Remarks:
- pH 6.8, DOC 17.3 mg/L, Ca 38.3 mg/L
- Effect conc.:
- 2.98 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Conclusions:
- Chronic zinc toxicity (expressed as 50 % effective concentrations [EC50]) varied up to 5-fold for the D. magna (21 d EC50 from 112 to 536 mg Zn/L). The determined NOEC range is 0.0626 - 0.491 mg Zn/L.
This result is also relevant for the target substance, which contains 16.5 % zinc ions. - Executive summary:
Chronic zinc toxicity to Daphnia magna (expressed as 50 % effective concentrations [EC50]) varied up to 5-fold (21-d EC50 from 112 to 536 mg Zn/L). The determined NOEC range is 0.0626 - 0.491 mg Zn/L.
This result is also relevant for the target substance, which contains 16.5 % zinc ions. Accounting to the Zn content of 16.5 %, this corresponds to a 21 d EC50 ranging from 0.68 to 3.25 mg/L, the NOEC (21 d) ranging from 0.38 - 2.98 mg/L.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- assessment report
- Duration:
- 9 d
- Dose descriptor:
- EC50
- Remarks:
- acclimation to 3 µg Zn/L for 10 generations
- Effect conc.:
- 2.15 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 9 d
- Dose descriptor:
- EC50
- Remarks:
- acclimation to 13 µg Zn/L for 10 generations
- Effect conc.:
- 2.35 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 9 d
- Dose descriptor:
- EC50
- Remarks:
- acclimation to 50 µg Zn/L for 10 generations
- Effect conc.:
- 2.72 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 9 d
- Dose descriptor:
- EC50
- Remarks:
- acclimation to 100 µg Zn/L for 10 generations
- Effect conc.:
- 2.96 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Conclusions:
- The chronic test results indicate that organisms acclimated to 50 and 100 µg Zn/L performed better (survival and reproduction). The EC 50 (9 d) values refering survival were 354 µg/L for organisms acclimised to 3 µg Zn/L, 387 µg/L for organisms acclimised to 13 µg Zn/L and 449 and 489 µg/L for organisms acclimised to 50 and 100 µg Zn/L, respectively.
This result is also relevant for the target substance, which contains 16.5 % zinc ions. - Executive summary:
In this study, the cladoceran Ceriodaphnia dubia was acclimated for 10 generations to four zinc concentrations ranging from 0 to 100 µg Zn/L and changes in zinc tolerance were monitored using chronic (9 days) assays. The chronic test results indicate that organisms acclimated to 50 and 100 µg Zn/L performed better (survival and reproduction). The EC 50 (9 d) values refering survival were 354 µg/L for organisms acclimised to 3 µg Zn/L, 387 µg/L for organisms acclimised to 13 µg Zn/L and 449 and 489 µg/L for organisms acclimised to 50 and 100 µg Zn/L, respectively. The reproduction and survival of organisms acclimated to 3 and 13 µg Zn/L was significantly lower than those acclimated to higher zinc concentrations. It can be concluded that culturing test animals in media lacking trace metals such as zinc could give rise to animals that are unnaturally sensitive to those same metals during toxicity tests.
This result is also relevant for the target substance, which contains 16.5 % zinc ions. Accounting to the Zn content of 16.5 %, this corresponds to an EC 50 (9 d) values refering survival of 2.15 mg/L for C. dubia acclimised to 3 µg Zn/L, 2.35 mg/L for C. dubia acclimised to 13 µg Zn/L and 2.72 and 2.96 mg/L for C. dubia acclimised to 50 and 100 µg Zn/L, respectively.
Referenceopen allclose all
The target substance contains 16.5 % Zn ions. Therefore, the hazard value for Zn ion is converted to the target substance considering a Zn content of 16.5 %.
EC50 pH 6.8 (Zn ion) = 0.536 mg/L
EC50 pH 6.8 (target substance) = 0.536/0.165 = 3.25 mg/L
EC50 pH 7.3 (Zn ion) = 0.112 mg/L
EC50 pH 7.3 (target substance) = 0.112/0.165 = 0.68 mg/L
NOEC pH 6.0 (Zn ion) = 0.0626 mg/L
NOEC pH 6.0 (target substance) = 0.0626/0.165 = 0.38 mg/L
NOEC pH 6.8 (Zn ion) = 0.491 mg/L
NOEC pH 6.8 (target substance) = 0.491/0.165 = 2.98 mg/L
The target substance contains 16.5 % Zn ions. Therefore, the hazard value for Zn ion is converted to the target substance considering a Zn content of 16.5 %.
EC50 acclimation to 3 µg Zn/L for 10 generations (Zn ion) = 0.354 mg/L
EC50 acclimation to 3 µg Zn/L for 10 generations (target substance) = 0.354/0.165 = 2.15 mg/L
EC50 acclimation to 13 µg Zn/L for 10 generations (Zn ion) = 0.387 mg/L
EC50 acclimation to 13 µg Zn/L for 10 generations (target substance) = 0.387/0.165 = 2.35 mg/L
EC50acclimation to 50 µg Zn/L for 10 generations (Zn ion) = 0.449 mg/L
EC50acclimation to 50 µg Zn/L for 10 generations (TP 1646) = 0.449/0.165 = 2.72 mg/L
EC50 acclimation to 100 µg Zn/L for 10 generations (Zn ion) = 0.489 mg/L
EC50 acclimation to 100 µg Zn/L for 10 generations (target substance) = 0.489/0.165 = 2.96 mg/L
Description of key information
Read-Across: WoE, Zn ion, D. magna, 21 d, EC50: 0.68 to 3.25 mg/L, NOEC: 0.38 - 2.98 mg/L (converted to the target substance taking into account the Zn content of 16.5 % at the most)
Read-Across: WoE, Zn ion, C. dubia, 9 d, EC 50: 2.15 - 2.96 mg/L (converted to the target substance taking into account the Zn content of 16.5 % at the most)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.38 mg/L
Additional information
Read-across Zn ion
De Schamphelaere et al., 2005
Chronic zinc toxicity to Daphnia magna (expressed as 50 % effective concentrations [EC50]) varied up to 5-fold (21-d EC50 from 112 to 536 mg Zn/L). The determined NOEC range is 0.0626 - 0.491 mg Zn/L.
This result is also relevant for the target substance, which contains 16.5 % zinc ions. Accounting to the Zn content of 16.5 %, this corresponds to a 21 d EC50 ranging from 0.68 to 3.25 mg/L, the NOEC (21 d) ranging from 0.38 - 2.98 mg/L.
Muyssen and Janssen, 2002
In this study, the cladoceran Ceriodaphnia dubia was acclimated for 10 generations to four zinc concentrations ranging from 0 to 100 µg Zn/L and changes in zinc tolerance were monitored using chronic (9 days) assays. The chronic test results indicate that organisms acclimated to 50 and 100 µg Zn/L performed better (survival and reproduction). The EC 50 (9 d) values refering survival were 354 µg/L for organisms acclimised to 3 µg Zn/L, 387 µg/L for organisms acclimised to 13 µg Zn/L and 449 and 489 µg/L for organisms acclimised to 50 and 100 µg Zn/L, respectively. The reproduction and survival of organisms acclimated to 3 and 13 µg Zn/L was significantly lower than those acclimated to higher zinc concentrations. It can be concluded that culturing test animals in media lacking trace metals such as zinc could give rise to animals that are unnaturally sensitive to those same metals during toxicity tests.
This result is also relevant for the target substance, which contains 16.5 % zinc ions. Accounting to the Zn content of 16.5 %, this corresponds to an EC 50 (9 d) values refering survival of 2.15 mg/L for C. dubia acclimised to 3 µg Zn/L, 2.35 mg/L for C. dubia acclimised to 13 µg Zn/L and 2.72 and 2.96 mg/L for C. dubia acclimised to 50 and 100 µg Zn/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
