1,4-bis(methoxymethyl)benzene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 29, 2020 to November 02, 2020

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

18 June 2019

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L
- Sampling method: samples were taken from test group and control group of fresh media at 0 hour fresh solutions and 96 hours old solutions.
- Sample storage conditions before analysis:

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.5007 g test item was weighed and dissolved in 5 L of test water by magnetic stirring for about 2 hours. After the test item was dissolved completely, the solution was used for exposure. 5 L test water was used for control group.

Test organisms (species):

other: Rare Minnow (Gobiocypris rarus)

Details on test organisms:

TEST ORGANISM
- Common name: Rare Minnow
- Strain: (Gobiocypris rarus)
- Source: CTI Chemical Safety Evaluation Research Center (SUZHOU) CO., LTD.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Weight at study initiation (mean and range, SD):
- Method of breeding:
- Maintenance of the brood fish:

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Twice on weekday and one time on weekend until 24 hours before the test was started
- Health during acclimation (any mortality observed): From hatching, there were not any signs of disease or significant numbers of mortality.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

40 to 250 mg/L (as CaCO3)

Test temperature:

23±2oC

pH:

6.0 to 8.5 pH units

Dissolved oxygen:

Not less than 60% of air saturation value (ASV) during the test period

Nominal and measured concentrations:

Range-finding test: 10 and 100 mg/L;
Definitive test: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Rectangle glass tanks (length: 30 cm, width: 20 cm, height: 30 cm)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration: Before usage, the water will be aerated to make dissolved oxygen concentration meet requirement of test.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: loading <0.8 g wet weight fish/L water

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light: 8 hours darkness and an intensity of 540-1000 lux
- Light intensity: 590 ~ 680 lux

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: no visible toxic signs.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

During the 96-hour exposure period, no mortality and visible toxicity were observed in the control group and test group of 100 mg/L.
During the test period, chemical analysis on test item concentration in test media by GC-MS/MS showed that for the test media of 100 mg/L, the measured concentration of fresh media was 95.2 mg/L. The measured concentration of old media was 93.9 mg/L. The mean concentration was 94.5 mg/L, which was 94.5% of nominal concentration.

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.

Executive summary:

A fish acute toxicity test of test item was conducted according to OECD 203 under GLP, with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period.

Based on the results of the preliminary test, the definitive test was carried out at 1 limit nominal concentration of 100 mg/L.

According to the results and based on the nominal concentration, the 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.

Description of key information

The 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

A fish acute toxicity test of test item was conducted according to OECD 203 under GLP, with Rare Minnow (Gobiocypris rarus) over a 96-hour exposure period.

Based on the results of the preliminary test, the definitive test was carried out at 1 limit nominal concentration of 100 mg/L.

According to the results and based on the nominal concentration, the 96-hour LC50 of the test item to Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L.