Allyltributylphosphonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study plan dated : 24. Apr. 2019 and experimental completion date 02. May. 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Allyltributylphosphonium chloride

EC Number:

216-231-7

EC Name:

Allyltributylphosphonium chloride

Cas Number:

1530-48-9

Molecular formula:

C15H32P.Cl

IUPAC Name:

tributyl(prop-2-en-1-yl)phosphanium chloride

impurity 1

Reference substance name:

Tetrahydrothiophene 1,1-dioxide

EC Number:

204-783-1

EC Name:

Tetrahydrothiophene 1,1-dioxide

Cas Number:

126-33-0

Molecular formula:

C4H8O2S

IUPAC Name:

tetrahydrothiophene 1,1-dioxide

impurity 2

Reference substance name:

Tributyl-1-propen-1-yl-phosphonium chloride

Molecular formula:

C15H32P.Cl

IUPAC Name:

Tributyl-1-propen-1-yl-phosphonium chloride

Test material form:

other: solid : wax like

Details on test material:

Homogeneity : homogeneous if totally melted
Note: For every use the test item was completely melted and the melted test item
was used for the test.

Specific details on test material used for the study:

Designation in Test Facility: 18032901G
Date of Receipt: 29. Mar. 2018
Condition at Receipt Room temperature, in proper conditions
Name Allyltributylphosphonium chloride
Batch no. INT 18 192
Appearance Beige crystallised mass

Homogeneity homogeneous if totally melted
Vapour pressure <0.1 hPa at 30 °C
Stability in solvents H2O: unknown; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Solubility in solvents H2O: > 1 g/L; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Production date not stated
Expiry date 17. Oct. 2019
Storage Room Temperature (20 ± 5°C)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The test item was stored in the test facility in a closed vessel at 14.0 – 24.8°C
For every use the test item was completely melted in a drying cabinet at 105°C.
A stock solution containing 100.0 mg/L test item in dilution water was prepared. The lower treatments were prepared by dilution of this stock solution with dilution water.
.

Test solutions

Vehicle:

not specified

Details on test solutions:

6.2 Positive Control
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201815R201). The 24h-EC50 value was determined as 1.48 mg/L. The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of po-tassium dichromate K2Cr2O7 as specified in the OECD guideline

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The keeping is performed similar to the method described in the OECD guideline, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12 from 02. Feb. 2015.
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20  2 °C

Study design

Test type:

static

Water media type:

saltwater

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hours

Test conditions

Hardness:

hardness in mmol/L:2.502 mg/L
hardness in mg CaCO3/L : 250 mg/L

Test temperature:

20.4 – 21.2°C

pH:

7.8

Dissolved oxygen:

The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.4 mg/L.

Details on test conditions:

7.3 Experimental Conditions
Date of performance 30. April – 02. May 2019
Treatments 1 / 2.2 / 4.6 / 10 / 22 mg/L
The concentrations to be tested are based on the result of a non-GLP pre-test.
Temperature 20.4 – 21.2°C
Duration 48 hours
Observation times 24 and 48 hours
Medium renewal none
Test vessels glass beakers, nominal volume 50 mL, tall shape
Replicates (Treatments) 4 vessels, each containing 20 ± 5 mL test solution and 5 daphnia
Replicates (Blank control) 4 vessels, each containing 20 ± 5 mL dilution water and 5 daphnia

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 3.53 mg/L

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

ca. 11.71 mg/L

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

2.2 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results were determined for the test itemAllyltributylphosphonium chloride(species:Daphnia magna).
 
48h-NOEC= 1.00 mg/L
48h-LOEC = 2.20 mg/L
24h-EC50= 11.71 mg/L
48h-EC50= 3.53 mg/L

According to CLP classification criteria, the substance is not classified as acute (short-term) aquatic toxicity

Executive summary:

One valid experiment was performed.

The study was performed using 5 concentrations ranging from 1 to 22 mg/L. For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.

All tested concentrations showed toxicity between 5 and 100% immobilisation. None of the animals was immobilised in the blank control.

Potassium dichromate K₂Cr₂O₇(CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.

 

At the beginning and at the end of the test, the content of the test item in the test solutions was determined usingLC-MS-determination. The measured concentrations lay between 101 % and 104 % of the nominal concentrations at the beginning of the test and between 113% and 120 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the nominal concentrations (cf. Guidance Doc. No. 23 §176).