trans-5-butyl-2-(3',3'',4'',5''-tetrafluoro-1,1':4',1''-terphenyl-4-yl)-1,3-dioxane

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 10, 2012 - Oct 08, 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)

Version / remarks:

1996

Deviations:

no

GLP compliance:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil: directly added

Test organisms (species):

Eisenia fetida

Animal group:

nematods

Details on test organisms:

TEST ORGANISM
- Common name: Earthworm
- Source: Nanjing Earthworm Nursery, China
- Age at test initiation (mean and range, SD): adult
- Mean Weight at test initiation: 384 mg

ACCLIMATION
- Acclimation period: 24 days

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

20 - 21°C

pH:

6.0 - 6.3

Moisture:

30- 36%

Details on test conditions:

TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 400 - 800 lux

EFFECT PARAMETERS MEASURED:
mortality, reaction on mechanical stimulus, behavioural and pathological symptoms assessed at days 7 and 14

TEST CONCENTRATIONS
- Range finding study: 10, 100, 1000 mg/kg artifical soil
- Main Study: 1000 mg/kg artificial soil

Nominal and measured concentrations:

Nominal concentration 1000 mg/kg dw

Reference substance (positive control):

yes

Remarks:

2-Chloroacetamide, purity >=98.5%, batch: 20120329; Sinopharm Chemical Reagent Co. Ltd.

Key result

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

yes

Conclusions:

A limit test on the acute toxicity of the test item was conducted according to OECD 207. The earthworm Eisenia fetida was used for the test in artificial soil. No mortality was observed within the test period. Therefore, the 14 d-LC50 was determined to be >1000 mg/kg dw.

Executive summary:

Study design

In this GLP study performed according to OECD GL 207 the acute toxicity of the test item in earthworms was investigated. During this test, the artificial soils was used with the moisture content of the test medium controlled in range of 30 - 36 % with pH values of 6 - 6.3. The test conditions were maintained at 20 - 21°C, a humidity of 80 - 85 % and continuous light with intensity of 400 - 800 lux.

Results

No dead earthworms were observed in treatments and control. During test all animals remained in soil. Based on the result of range-finding test and the Limit Test, the 14 d-LC50 of the test item in the artificial soil was determined to be greater than 1000 mg/kg dry weight soil. The highest concentration causing no mortality (14 d-LC0) was 1000 mg/kg dry weight soil.

Conclusion

No mortality was observed within the test period. Therefore, the 14 d-LC50 was determined to be >1000 mg/kg dw.

Description of key information

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 207. The earthworm Eisenia fetida was used for the test in artificial soil. No mortality was observed within the test period. Therefore, the 14 d-LC50 was determined to be >1000 mg/kg dw.