(6-Bromo-2-pyridinyl)(4-methylphenyl)methanone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 1985 - Febraury 1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

Principles of method if other than guideline:

The test was largely carried out as described by Ames:
Amea,B.N.; J, McCenn and e:. Yamasaki. Methods for detecting carcinogens and mutagens with the Selmonella/Mammallan-Microsome Mutagentclty Test.
Mut. Res..31, 347-364, 1975

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-bromo-6-(4-methylbenzoyl)pyridine

EC Number:

618-079-0

Cas Number:

87848-95-1

Molecular formula:

C13H10BrNO

IUPAC Name:

2-bromo-6-(4-methylbenzoyl)pyridine

Specific details on test material used for the study:

(E)-Ethyl 3-(6-(4-toluoyl)-2-pyridyl)acrylate
Batch WDCP/78/24/178/1
White/buff powder.

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 1538

Metabolic activation:

with and without

Metabolic activation system:

liver microsome prepantlon (S9)

Controls

Controls 1

Positive control substance:

other: Sodium nitrite

Controls 2

Positive control substance:

other: 2-Aminoanthracene

Controls 3

Positive control substance:

9-aminoacridine

Controls 4

Positive control substance:

other: Hycanthone

Controls 5

Positive control substance:

other: Daunomycin

Controls 6

Positive control substance:

other: Azaserine

Controls 7

Untreated negative controls:

yes

Results and discussion

Test results

Test results 1

Key result

Species / strain:

S. typhimurium TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 2

Key result

Species / strain:

S. typhimurium TA 1537

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 3

Key result

Species / strain:

S. typhimurium TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 4

Key result

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Test results 5

Key result

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Any other information on results incl. tables

The test article did not induce a twofold increase in the number of revertants for any of the tester strains, with or without the liver microsome preparation (S9) in either test. It is therefore concluded that ((E)-Ethyl 3-(6-(4-toluoyl)-2-pyridyl)acrylate is non-mutagenic at concentrations up to and including that of 50 µg/plate (18.5 µg/cm3) in the first test in the presence and absence of a liver microsome preparation (S9), and In the second test in the absence of S9; and 158 µg/plate (58.5 µg/cm3) in the second test in the presence of S9. Above these concentrations, reduced background growth of tester strains indicated toxicity of the compound precluding further evaluation of results.

Applicant's summary and conclusion

Conclusions:

Substance was determined not to be mutagenic, observed in all strains at the concentrations tested.