4H-Pyrazino[2,1-a]isoquinolin-4-one, 2-acetyl-1,2,3,6,7,11b-hexahydro-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004-12-14 to 2005-04-06

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples were taken after preparation of the test medium and at the end of the experimental part.

Vehicle:

no

Details on test solutions:

The water was composed according to ELENDT M4 (1990)
For the reconstituted water the following chemicals (analytical grade) were dissolved in fully demineralized water:
Macro nutrients mg/L
CaC12.21120 293.8
MgSO4.7H2O 123.3
NaHCO3 64.8
KC1 5.8
Na2SiO39H2O 10
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18

Trace elements, macro nutrients and vitamins were added to freshly prepared ISO-medium to reach the following concentrations:

Trace elements mg/L
B 0.5
Fe 0.2
Mn 0.1
Li,RbandSr 0.05
Mo 0.025
Br 0.0125
Cu and Zn 0.0063
Co and J 0.0025
Se 0.0010
V 0.0003
Na2EDTA • 2H20 2.5

Vitamins µg/L
Thiamine 75.0
B12 1.0
Biotin 0.75
After preparation the reconstituted water was aerated for 24 hours.
Hardness: 250 mg/L expressed as CaCO3. pH: 7.9 ± 0.3 after aeration for 24 hours.

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna (Straus)
Breeder: Testf Facility
Age: Daphnia magna, not older than 24 hours.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

14° dH (abount 250 mg/L CaCO3)

Test temperature:

21 - 22 °C

pH:

7.9 +/- 0.3

Dissolved oxygen:

> 90 %

Salinity:

n.a.

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

The test medium (reconstituted water and test material) was freshly prepared. Therefore, the calibrated flask with test material and vehicle, reconstituted water, was shaken. The solution was used for the study.

At the start of the experimental phase, 5 Daphnia magna were placed into 10 mL of reconstituted water (control group) or test medium (test material group). The Daphnia magna were not fed, and the control medium and test medium were not aerated during the test.
The test vessels were labeled to assure an unequivocal identification.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

not applicable

Table 1 Result overview

Concentration Test item	Immobilization immobilized /exposed
mg/L	24 hours	48 hours
0 (control)	0/20	0/20
100	0/20	0/20

Validity criteria fulfilled:

yes

Conclusions:

An aqueous solution of 100 mg/L of the test item revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna was > 100 mg/L (limit test) and could not be determined in this test.

Executive summary:

The acute toxicity of the test material was evaluated in a static test system with Daphnia magna in accordance with OECD TG 202. Juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four vessels per concentration containing five Daphnia magna each, i.e., 20 Daphnia per concentration (test medium group). The limit of quantification of the analytical method was about 0.5 mg/L. Daphnia magna were exposed to a test material concentration of 100 mg/L, (limit-test) in an open static system. The analytically determined concentration was about 102 % at the start and 101 % at the end. So the nominal concentration is regarded as equivalent to the analytical concentration. For the test material, the following EC50 values for Daphnia magna were determined:

24 h EC50 > 100 mg/L

48 h EC50 > 100 mg/L

In conclusion, an aqueous solution of 100 mg/L of the test item revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna was > 100 mg/L (limit test) and could not be determined in this test.

Description of key information

Short term toxicity to daphnia was assessed in an OECD TG 202 compliant study and 48 h EC50 was greater than 100 mg/L (reference 6.1.3-1).

Additional information

The acute toxicity of the test material was evaluated in a static test system with Daphnia magna in accordance with OECD TG 202. Juvenile Daphnia magna were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four vessels per concentration containing five Daphnia magna each, i.e., 20 Daphnia per concentration (test medium group). The limit of quantification of the analytical method was about 0.5 mg/L. Daphnia magna were exposed to a test material concentration of 100 mg/L, (limit-test) in an open static system. The analytically determined concentration was about 102 % at the start and 101 % at the end. So the nominal concentration is regarded as equivalent to the analytical concentration. For the test material, the following EC50 values for Daphnia magna were determined:

24 h EC50 > 100 mg/L

48 h EC50 > 100 mg/L

In conclusion, an aqueous solution of 100 mg/L of the test item revealed no aquatic toxicity in the test system. The 48 hours EC50 to Daphnia magna was > 100 mg/L (limit test) and could not be determined in this test.