7,17,28,38-tetraazatridecacyclo[24.16.2.2²,⁵.1⁸,¹².1²⁹,³³.0³,²².0⁴,¹⁹.0⁶,¹⁷.0²³,⁴³.0²⁷,³⁸.0⁴⁰,⁴⁴.0¹⁶,⁴⁶.0³⁷,⁴⁵]octatetraconta-1(42),2(48),3,5(47),6,8,10,12(46),13,15,19,21,23,25,27,29(45),30,32,34,36,40,43-docosaene-18,39-dione; 7,17,28,38-tetraazatridecacyclo[24.16.2.2²,⁵.1⁸,¹².1²⁹,³³.0³,²².0⁴,¹⁹.0⁶,¹⁷.0²³,⁴³.0²⁸,³⁹.0⁴⁰,⁴⁴.0¹⁶,⁴⁶.0³⁷,⁴⁵]octatetraconta-1(42),2(48),3,5(47),6,8,10,12(46),13,15,19,21,23,25,29,31,33,35,37(45),38,40,43-docosaene-18,27-dione

Endpoint summary

Additional information

The test substance is with high probability acutely not harmful to aquatic organisms.

The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations.

The test substance belongs to the category of perylene as indicated by its chemical core structure. Based on this structure and the chemical and biological stability of perylenes under environmental conditions, data from structural analogue perylenes can be used to cover this endpoint required under REACh if no data for the original test substance is available.

 

The acute toxicity of the substance to Brachydanio rerio was determined in a GLP limit test according to OECD guideline 203. A static test system was set up according to the guideline and the fish were exposed for 96 hours. Based on nominal test substance concentrations an LC50 > 100 mg/l was determined (BASF AG 2005). These results are supported by another GLP guideline study which was conducted under similar test conditions as the key study. At test termination after 96 h a LL50 > 100 mg/L (nominal) was determined (BASF China 2012).

The toxicity of Perylen Schwarz/Black II to Daphnia magna was examined in a 48 hour static test according to OECD guideline 202. At test termination an EC50 of > 100 mg/l based on nominal test substance concentrations was determined (BASF AG 2006).

The inhibiting abilities of Perylen Schwarz/Black II to Pseudokirchnerella subcapitata were examined in a GLP study according to OECD guideline 201. After 72 h the EC50 value for Perylen Schwarz/Black II was determined to be > 100 mg/L based on the nominal concentration of the test substance (BASF AG 2006). No effect was observed up to the solubility limit of the test substance in water.

The acute toxicity of the analogue substance CAS 81-33-4 to aquatic plants has been examined in a GLP guideline study according to OECD 221 with the test species Lemna gibba (BASF SE, 2012). At test termination after 7 d, an ECr50 > 0.007 mg/L (measured) relating to growth rate (frond rate and dry weight, respectively) was determined.

The effect of the substance on the respiration rate of activated sludge was determined in a GLP study according to OECD guideline 209. The respiration rate was monitored for 180 minutes. Based on the nominal test substance concentrations an EC20 of > 996 mg/l (EC50 > 996 mg/l) was determined (BASF AG 2006).

All tests were run with concentrations above the water solubility of the test substance and its analogues, respectively. Thus, no effects in the range of the water solubility of the substance were determined.

 

The hazard assessment of the substance reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance, nor are there any further indications that the substance may be hazardous to the environment. Therefore, and for reasons of animal welfare long-term toxicity testing in fish and aquatic invertebrates is not provided.