1-Octadecanaminium,N,N-Dimethyl-N-Octadecyl-,(Sp-4-2)-[29h,31h-Phthalocyanine2-Sulfonato(3-)-N 29,N 30,N 31,N 32]Cuprate(1-)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study; well documented study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

-Stable under storage conditions
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Saturated solution was prepared: 20.1 mg of test substance was quantitatively added to 2000 ml medium providing a stock solution of 10 mg/l. The oversaturated solution was stirred for 72 hours. From this solution 2 x100 ml was taken and filtered over a 45 um filter and as such tested. Another 2x 100 ml from the stirred stock solution was tested without filtration.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: (Crustacea, Cladocera) (Straus, 1820)
- Source: In house
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding: Synchronously in DSW with a density of 25 Daphnia per liter in a climate room
- Feeding during test: No feeding
ACCLIMATION
- Type and amount of food: A suspension of fresh water algae
- Feeding frequency: : Once daily

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

24 h

Test conditions

Hardness:

11.7 0 DH

Test temperature:

18.5 - 19.5 C

pH:

8.3 - 8.4

Dissolved oxygen:

> 5 mg/L

Nominal and measured concentrations:

10 mg/L nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 mL glass vessel
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): duplicate
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dutch standard water (DSW)
- Chlorine: 96.3 mg/l
- Intervals of water quality measurement: At the beginning and end of the test
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

exceeding solubility

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/LC50: 1.48 mg/l
- Other: The reference substance was tested two days before the final test with the test material using Daphnia from the same culture.

Any other information on results incl. tables

Chemical analysis revealed that due to the very low solubility of the test material in water and interference of compounds present in tap-water with the analysis, no detectable levels of the test material could be recorded. Therefore it was decided not to perform analysis of test concentration during the final test.

 

After 24 hours of exposure no immobilization was seen in either the filtered or the unfiltered oversaturated solution.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this study the 24 hour EC50 in Daphnia magna was greater than the saturated concentration of the test material when tested as an oversaturated solution of 10 mg/L.

Executive summary:

Daphnia were exposed for 24 hours in a static system to an oversaturated concentration of 10 mg/l of the test material in tap-water. After 24 hours of exposure no immobilization was observed, The 24 hour EC50 in Daphnia magna was greater than the saturated concentration of the testmaterial when tested as an oversaturated solution of 10 mg/L.