1-Octadecanaminium,N,N-Dimethyl-N-Octadecyl-,(Sp-4-2)-[29h,31h-Phthalocyanine2-Sulfonato(3-)-N 29,N 30,N 31,N 32]Cuprate(1-)

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed according to guidline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

yes

Remarks:

The temperature of the test solution was slightly higher (23.0 and 22.5 C) than recommended in the guideline on two occasions. However these occurrences were brief in duration and were recorded on days 0 and 21 and not considered to have adverse effects

GLP compliance:

yes

Test material

Test material information

Constituent 1

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fraction was prepared by stirring the appropriate quantities of the test material in Daphnia test media for 20 hours, followed by settling period of 4 hours. The test solutions were filtered before exposure of the Daphnia.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
-Source: Reading University
- Age of parental stock (mean and range, SD): Less than 24 hours
- Food type: Chorella vulgaris
- Amount: 1 mg Organic carbon/litre of culture water
- Frequency: each working day

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

250 mgCaCO3/L

Test temperature:

19.5 to 23 C

pH:

6.7 to 8.0

Dissolved oxygen:

79 to 100% ASV

Nominal and measured concentrations:

1.0, 3.2, 10, 32, 100 mg/L (nominal)

Details on test conditions:

-Aeration: A wide bore glass tube was used to transfer on Daphnia, in a minimum amount of culture water (<0.5ml) into ten replicate 100ml glass beakers. The test vessels were not aerated during the test period. To limit aerial contamination of the test vessels and reduce evaporation losses, a transparent Perspex sheet was placed over the tops of the beakers.
- No. of organisms per vessel: one
- No. of vessels per concentration (replicates): ten replicate
- No. of vessels per control (replicates): ten
-Intervals of water quality measurement: The dilution water was reconstituted water (ISO Test Media) from Annex 3 of OECD guideline 202. The pH was adjusted to 8.0 +/- 0.2 using 1M NaOH or 1 M HCl. The total hardness was approximately 250 mg CaCO3/L.

OTHER TEST CONDITIONS
- Adjustment of pH: There was no adjustment of the pH during the study.
- Photoperiod: sixteen hours light and eight hours dark
- Light intensity: not exceeding 1500lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

84.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 35.4 to > 100 mg/L

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 5

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Reported statistics and error estimates:

The LOEC and NOEC for fecundity were calculated by Dennett’s Test
Daphnia survival were calculated by Fisher’s Exact Test.

Any other information on results incl. tables

The mortality of the parent Daphnia and the number of offspring produced by each parent animal were recorded every day. The presence, if any of aborted eggs or dead offspring were also recorded and removed along with the neonates.

Summary of Information on reproduction and mortalities

Concentration (mg/l)	Control	1.0	3.2	10	32	100
Number of adult mortalities	1	2	1	4	3	3
Total number offspring per conc.	809	832	602	480	409	280
Meran number of broods per adult*	4.8	4.3	4.0	3.7	2.7	3.8
Mean number offspring per brood	17.9	20.5	15.3	22.8	16.7	10.6
Number of days to first brood	8-11	8-13	8-12	11-14	12-14	9-15
Number of days to second brood	2-3	2-6	3-6	2-3	2-3	2-5
Number of aborted broods per conc	0	0	0	0	0	0
Number of males per conc.	0	0	0	0	0	0

*Where the number of offspring in brood =3 or less, not counted as a full brood for determination of the mean number of broods.

The table below shows the number of adults alive and the mean number of offspring per adult produced for each test concentration.

 

	Nominal Concentration (mg/l)
	Control	1.0	3.2	10	32	100
Number of adults alive	9	8	9	6	7	7
Mean number of offspring per surviving adult	89.9	104.0	66.9	81.7	58.4	40.0

 

One (10%) of the ten control parentDaphniadied during the study and noDaphniawere trapped at the surface of the water. The number of live offspring produced per parent animal surviving to the end of the test in the control vessels was 69-104 with a mean value of 89.9. The coefficient of variation for the control fecundity was calculated as 11.7%

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test material had an EC50 of 84.6 mg/l WAF with a NOEC of 10 mg/l WAF and a LOEC of 31mg/l WAF for reproduction. The NOEC and LOEC for parent Daphnia survival was 100mg/L WAF (highest concentration tested)

Executive summary:

Reproduction

Using the parameter of fecundity, that is live offspring per parentDaphniaalive at the end of the test the 21-Day reproduction EC50 value of the test material was estimated to be 84.6 mg/L WAF. The highest no observed effect concentration (NOEC) after 21 days was 10 mg/l WAF. The lowest observed effect concentration (LOEC) after 21 days was 32 mg/l WAF. The LOEC and NOEC were calculated by Dennett’s Test

Parent Mortality

The 21-Day NOEC for parentDaphniasurvival was calculated as 100 mg/L WAF. The 21-day LOEC for parent Daphnia survival was calculated as greater than 100 mg/L WAF. Both the NOEC and LOEC calculations for parentDaphniasurvival were calculated by Fisher’s Exact Test.