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EC number: 604-636-5 | CAS number: 148477-71-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Details on sampling:
- Earthworms were exposed to test material at 1000 mg a.i/kg dw of the soil.
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- The artificial soil consists of 69 % fine quartz sand (84 % of the sand has a particle size of 0.06 - 0.2 mm), 10 % dried, finely ground peat (sphagnum peat; pH 2 - 4), 20 % kaolin (kaolinite content of about 36 %, pH value approximately 7, "Kaolin W", from Erbsloh / Giesenheim) and around 1% calcium carbonate (pure) to adjust the pH value to 6 ±0.5 (figures refer to dry weight). The test soil was first of all mixed dry from these components in a plough-share mixer, and moistened with some of the final volume of water required.
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- Age at test initiation: over two months old
Weight at study initiation: 0.36 g. Range in control group 0.30 to 0.50 g.
Source: laboratory culture. Original source Prof. Graff, 38104 Braunschweig, Germany.
Acclimatization: The earthworms were taken from a laboratory culture maintained for several years. Culture conditions were 22 ± 2 °C, 70 to 90 % humidity, 12:12 light/dark cycle. The worms were removed from the culture and placed in test soil on the day prior to the study. The test conditions were very similar to the test conditions.
No specifics on health of the organisms - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Total exposure duration:
- 14 d
- Test temperature:
- Temp: 20 ± 1°C
- pH:
- pH = 5.6 at initiation; 5.8 - 5.9 at
termination.
100 mL deionized water to 500 g
dry weight soil - Moisture:
- Target for artificial soil was 35%
when the worms were introduced.
Initiation: 24 - 25%
Termination: 33% - Details on test conditions:
- Soil: Freshly mixed artificial soil.
Test container: 1.5 liter glass jars with glass lids. Each container had 500 g dry weight soil.
No. of replicates: Control and 1000 mg ai/kg dry wt. soil
Per treatment group: Four replicates
Per control: Four replicates
No. of earthworms per treatment: 10 earthworms per replicate; 40 worms per
treatment.
Co-solvents used or not (if yes
report the name and concentration): no solvents used
Test conditions:
Temp: 20 ± 1°C
continuous light at 400 to 800 lux
70-90% humidity
25 - 34 % soil moisture
Stability and homogeneity of test material in the medium?
A pre-check was done with 14C labeled compound to prove the mixing process was
homogenous and reproducible.
Stability was not a required element of this 14 day test.
Observation interval: Day 0, Day 7 and Day 14
Parameters measured including the sublethal effects/toxicity symptoms: Mortality, weight, toxicity symptoms
Raw data included: Yes
Definitive study:
The artificial soil consists of 69 % fine quartz sand (84 % of the sand has a particle size of 0.06 - 0.2 mm), 10 % dried, finely ground peat (sphagnum peat; pH 2 - 4), 20 % kaolin (kaolinite content of about 36 %, pH value approximately 7, "Kaolin W", from Erbsloh / Giesenheim) and around 1% calcium carbonate (pure) to adjust the pH value to 6 ±0.5 (figures refer to dry weight). The test soil was first of all mixed dry from these components in a plough-share mixer, and moistened with some of the final volume of water required.
Range-finding Study: A pre-study was conducted using the concentrations of control, 0.1, 1,
10, 100 and 1000 mg ai/kg dw soil. No mortality was noted at any concentration, except the 1
mg ai/kg dw soil which had 5%. The weight alteration across all treatments was from -2 to 5 %.
There were no adverse effects at any concentration. The pre-study was conducted with two
replicates of 10 earthworms at each concentration. - Nominal and measured concentrations:
- Nominal: control and 1000 mg ai/kg dry
wt. soil.
No analysis was done. This is not a
required element of the test.
A pre-check was done with 14C labeled
compound to prove the mixing process was
homogenous and reproducible - Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide at 10, 18, 24, 32 and 56 mg/kg. The 14 day LC50 was 33 mg/kg dw soil (95% CI = 31 to 35 mg/kg)
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 1 000 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- There were no mortalities (0%) in the controls and in the treatment. The weight alteration in the
controls was -12% and in the treatment was -15%. There were no significant differences from
the control for the 1000 mg ai/kg dw soil treatment. - Reported statistics and error estimates:
- No statistics were needed for evaluation of mortality and toxic symptoms since none (0%) were
observed during the 14-day study.
The weight alterations of the test organisms were statistically evaluated by the U-Test of
Wilcoxon, Mann & Whitney (Probability level P = 0.05, two sided). - SUB-LETHAL TOXICITY ENDPOINTS:
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 14 day EC50 and LC50 were greater than 1000 mg ai/kg dw soil. There were no adverse
effects noted in this study for mortality, toxic symptoms or weight alteration.
NOEC = 1000 mg ai/kg dw soil
LOEC = > 1000 mg ai/kg dw soil - Executive summary:
In a 14-day acute toxicity study, earthworms {Acini fetida} were exposed to spirodiclofen technical at 1000 mg a.i./kg dry weight of soil. The reference chemical used was chloroacetamide at 10, 18, 24, 32 and 56 mg a.i/kg dw of the soil. The experiment was carried out in accordance with OECD 207. The 14-day LC50 was > 1000 mg a.i/kg dw of soil. The 14-day NOEC, based on all endpoints was 1000 mg a.i./kg dw of soil. The LOEC, based on all endpoints was greater than 1000 mg a.i./kg dw of soil. The technical a.i. is considered to be non-toxic to earthworms up to and above a concentration of 1000 mg a.i./kg dw of soil.
There was no mortality, no toxic symptoms and no significant difference in weight of the earthworms in the 1000 mg ai/kg dw soil.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with earthworms.
Reference
Table 4: Effect of spirodiclofen technical on mortality of Acini fetida.
Treatment (mg a.i./kg dw soil) [nominal conc.] | Observation period | ||||||
Day 0 | Day 7 | Day 14 | |||||
No. Dead | % mortality | No. Dead | % mortality | No. Dead | % mortality | ||
Control | 0 | 0 | 0 | 0 | 0 | 0 | |
1000 | 0 | 0 | 0 | 0 | 0 | 0 | |
NOEC | 1000 mg ai/kg dw soil. | ||||||
LOEC | > 1000 mg ai/kg dw soil. | ||||||
LC50 | > 1000 mg ai/kg dw soil. | ||||||
Reference chemical | LC50: | chloroacetamide at 10, 18, 24, 32 and 56 mg/kg. The 14 day LC50 was 33 mg/kg dw soil (95% CI = 31 to 35 mg/kg) |
Table 5: Sub-lethal effect of spirodiclofen technical on Acini fetida. Average of 4 replicates used for the study.
Treatment (mg a.i./kg soil) [nominal conc.] | Observation period | |||
Day 0 | Day 14 | |||
weight (g) | % loss | weight (g) | % loss | |
Control | 0.36 | - | 0.32 | 12 |
1000 | 0.36 | - | 0.31 | 15 |
NOEC | 1000 mg ai/kg dw soil | |||
LOEC | > 1000 mg ai/kg dw soil | |||
EC50 | > 1000 mg ai/kg dw soil |
Description of key information
In an acute tox study with, Eisenia fetida, were exposed to a concentration of 1000.0 mg a.s./kg soil dry weight (dw). The EC50 was determined to be 1000 mg/kg soil (dw).
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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