Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 610-461-5 | CAS number: 495-61-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch name: 1806PD02BBI9000
Purity: 94.1% (main component), according to certificate of analysis
Certificate of Analysis Date: June 30, 2021
Aggregate State at Room Temperature: Liquid
Colour: Colourless – yellowish
Theoretical Oxygen Demand: ThOD NH4: 3.288 mg oxygen per mg test item
Expiry Date: October 21, 2022
Storage Conditions at the Test Facility: At 4 +/- 4 °C, in the dark
Safety Precautions: Routine safety and hygienic procedures - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge, microorganisms from a domestic waste water treatment plant will be supplied by a municipal sewage treatment plant (e.g. Rossdorf, Germany).
- Duration of test (contact time):
- 60 d
- Initial conc.:
- 41.6 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The closed test flasks will be incubated in a climatic chamber under continuously stirring. Evolving carbon dioxide will be absorbed in an appropriate absorber. The consumption of oxygen will be determined by measuring the change of pressure in the flasks.
Preparation of Test Flasks: The amounts of test item and reference item will be directly weighed into the test flasks. No emulsifiers or solvents will be used.
Test duration: 28 days, extended to 60 days
Surrounding Type: Climatic chamber
Temperature: 22°C ± 1°C
Light Conditions: Darkness
pH Value: 7.4 ± 0.2 - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 33
- Sampling time:
- 60 d
- Results with reference substance:
- 78% after 28 days of exposure.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
Reference
Description of key information
Based on GLP-study according to OECD 301F, the test substance is considered as "not readily biodegradable" according to OECD criteria (33% after 60 days).
Key value for chemical safety assessment
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
