1,3,4-Octadecanetriol, 2-amino-, sulfate (2:1) (salt), D-ribo- (8CI)

Melting point / freezing point

Administrative data

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2021-03-02 to 2021-03-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2021

Report date:

2021

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 102 (Melting point / Melting Range)

Version / remarks:

adopted on 27 July 1995

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method A.1 (Melting / Freezing Temperature)

Version / remarks:

Council Regulation (EC) No 440/2008; May 30th 2008

GLP compliance:

no

Type of method:

differential scanning calorimetry

Test material

Test material information

Constituent 1

Reference substance name:

(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-aminium sulfate

EC Number:

917-270-9

Cas Number:

29790-50-9

Molecular formula:

C36H80N2O10S

IUPAC Name:

(2S,3S,4R)-1,3,4-trihydroxyoctadecan-2-aminium sulfate

Test material form:

solid

Specific details on test material used for the study:

The physical state of the test item at 20 °C and 101.3 kPa is a white, crystalline solid. All analyses included in this report have been performed with the batch EP18B05521, which passed the quality control measurements without any irregularity previously. All specifications were met.

Results and discussion

Melting / freezing point

Key result

Decomposition:

yes

Decomp. temp.:

ca. 145 °C

Any other information on results incl. tables

Chemically, it is a salt consisting of two molecules of Phytosphingosine and one molecule of sulphuric acid.

The melting temperature of Phytosphingosine (EC 439-210-6, CAS 554-62-1) itself was determined to be 101 °C (374 K) by DSC (differential scanning calorimetry). Reaction or decomposition of the test substance started at 148 °C (421 K). Boiling of Phytosphingosine was not observed below the temperature at which reaction or decomposition started.
Melting and boiling temperatures of salts are usually higher.
Experiments
To verify the statement above, the thermic behaviour was determined using a melt microscope (MP80, Mettler-Toledo) with a boiling point capillary.
The sample was heated from room temperature up to 200 °C with a rate of 5 °C/min.
Up to 135 °C the sample remains white and powdery. Then the sample lumps together. Starting from approx. 145 °C the sample discolours continuously. Finally, at 200 °C the colour is medium brown.
No clear melting point and bubbles were observed.

Result
No melting temperature and no boiling temperature are observed below the temperature at which reaction or decomposition started (approx. 145 °C).
Probably small impurities cause the test item to clump.

Applicant's summary and conclusion

Conclusions:

No melting temperature is observed below the temperature at which reaction or decomposition started (approx. 145 °C).

Executive summary:

The thermic behaviour was determined using a melt microscope (MP80, Mettler-Toledo) with a boiling point capillary.
The sample was heated from room temperature up to 200 °C with a rate of 5 °C/min.
Up to 135 °C the sample remains white and powdery. Then the sample lumps together. Starting from approx. 145 °C the sample discolours continuously.
No clear melting point and bubbles were observed.