Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2015-02-13 to 2015-04-28
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
Version / remarks:
January 21, 2000
GLP compliance:
Specific details on test material used for the study:
The test material is a white solid.
Analytical monitoring:
Details on sampling:
A sample of each replicate of each treatment was taken at intervals of 3 hours, 7, 14, 28 and 42 days and the mineral nitrogen content of the soil was determined.
Details on preparation and application of test substrate:
200 g soil dry weight (= one sub-sample) per test vessel was weighed. The soil was mixed with 0.5% (i.e. 1.0 g/200 g soil d.w.) lucerne meal by means of a hand-stirrer (the C/N ratio of the lucerne meal was 13.2/1, see Appendix 1). One additional soil sample (without lucerne meal) was used for determination of the initial NO3-N-content. The initial NO3-N-content was 4.45 mg /100 g soil d.w. Since the water solubility was very low, the test item was thoroughly mixed with quartz sand. Subsequently the obtained mixture was added and mixed with the soil by means of a hand stirrer. For an optimum distribution of the test item in the soil, the test item quartz sand mixture was applied at a ratio of about 10 g per kilogram soil dry weight. Water was added to the soil to achieve a water content of approximately 45% of WHC (see Appendix 2). The incubation of the prepared soil was carried out in wide mouth glass flasks (500 mL) under the conditions mentioned above. The screw caps of the flasks used permitted an air exchange. The water content of the soil in each test vessel was determined at test start (after application) and adjusted once a week to the required range of 40 - 50% of WHC (see Appendix 4). The pH-values of the soil used in the test were measured at test start (after application) and at the sampling on day 28 and 42 (see Appendix 4).
Test organisms (inoculum):
other: sandy loam or loamy sand
Total exposure duration:
42 d
Test temperature:
19.2 - 20.7°C
18.21 - 19.54 g/100 g soil d.w. (equivalent to 46.04 - 49.42% of WHC)
Details on test conditions:
- Test container (type, material, size): glass flasks (500 mL)
- Amount of soil: 200 g soil dry weight (= one sub-sample) per test vessel.
- No. of replicates per concentration: 3 replicates
- No. of replicates per control: 3 replicates

- Method: The incubation of the soil samples was performed as a series of individual and equally sized sub-samples of each treatment group.

- Geographical reference of sampling site (latitude, longitude): 12.694435960 degrees East, 51.403774567 degrees North
- Depth of sampling: 20 cm
- Soil texture
- % sand: 54.3 (USDA), 52.9 (DIN 11277)
- % silt: 35.1 (USDA), 36.6 (DIN 11277)
- % clay: 10.6 (USDA), 10.6 (DIN 11277)
- Soil classification system: sandy loam according to USDA or loamy sand according to DIN 11277.
- pH (in water): 6.1
- Maximum water holding capacity (in % dry weigth): 39.54 g/100 g soil d.w.
- Cation exchange capacity (mmol/kg): 11.2 cmol+ /kg soil
Nominal and measured concentrations:
Nominal concentration: 2 and 10 mg a.s./kg soil dry weight
Reference substance (positive control):
Key result
42 d
Dose descriptor:
Effect conc.:
>= 10 mg/kg soil dw
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
nitrate formation rate
Details on results:
Nitrogen transformation
The findings are summarised in the table below. Values are given as mg NO3-N/kg soil d.w. The test item pencycuron a.s. caused a temporary stimulation of the daily nitrate rate at the tested concentrations of 2 mg a.s./kg dry soil and 10 mg a.s./kg dry soil at time interval 14-28 days after application. However, no adverse effects of pencycuron a.s. on nitrogen transformation in soil could be observed at both tested concentrations at the end of the test, 42 days after application (time interval 28-42). Differences from the control of -0.4% (test concentration 2 mg a.s./kg dry soil) and -0.8 % (test concentration 10 mg a.s./kg dry soil) were measured at the end of the 42-day incubation period (time interval 28-42). For details see Table 2.
Results with reference substance (positive control):
In the most recent test with the toxic standard, Dinoterb caused an effect of +39.1%, +62.5% and +112.0% (required =25%) on the nitrogen transformation in a field soil at the tested concentrations of 6.80 mg, 16.00 mg and 27.00 mg Dinoterb per kg soil dry weight, respectively, 28 days after application (time interval 14-28) and thus demonstrates the sensitivity of the test system
Validity criteria fulfilled:
The coefficients of variation in the control for NO3-N were maximum 7.8% and thus fulfilled the demanded range (=15%).
Pencycuron a.s. caused no adverse effects (difference to control <25%, OECD 216) on the soil nitrogen transformation (expressed as NO3-N-production) at the end of the 42-day incubation period. The study was performed in a field soil at concentrations up to 10 mg a.s./kg dry soil weight.
Executive summary:

At the end of the 42-day incubation period, Pencycuron a.s. caused no adverse effects (difference to control <25%, OECD 216) on the soil nitrogen transformation (expressed as NO3-N-production). No effects (effects <25%) was observed at 10 mg a.s./kg dws after 42 days of incubation.

Description of key information

The purpose of this study was to determine the effects of Pencycuron on the activity of soil microflora with regard to nitrogen transformation in a laboratory test. A loamy sand soil was exposed for 42 days to 2.0 and 10.0 mg/kg soil dw. Pencycuron caused no adverse effects (difference to control <25%) on the soil nitrogen transformation (measured as NO3-N production) at the end of the 42-day incubation period. The NOEC was determined to be ≥10.0 mg a.s./kg soil dw.


Test species




Sandy loam soil

42-d NOEC ≥10.0 mg a.s./kg soil dw

Key study

Schulz (2015)

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:
10 mg/kg soil dw

Additional information