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Short-term toxicity to aquatic invertebrates

Description of key information

EC₅₀ (48h) = 0.262 mg/l (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

0.262 mg/L

Additional information

This study was performed to assess the potential toxic effects of the test item to Daphnia magna, under static conditions (Test Guideline OECD 202).

Based on results of the preliminary range finding study, concentration levels selected for the main study were 0.05, 0.09, 0.16, 0.28, and 0.50 mg test item/L, along with control and vehicle control. In the main study, 48-h static toxicity test procedure was followed, comprising six groups. Each group consisted of four replicates with 5 daphnids per replicate. Daphnids were exposed to 16h light and 8h dark cycle. Temperature, pH, and dissolved oxygen content of reconstituted water were recorded at 0 h and 48 h. Total hardness of reconstituted water was measured once during the acclimatisation and test period. 

Test item in the test media was found to be stable up to 48 h (>80% of the nominal concentration). Test media was analysed for concentration and stability at 0 and 48 h during the main study and was within the acceptable limit (>80% of the nominal concentration).

The highest tested concentration level of the test item causing no immobilisation was 0.09 mg/L and the lowest tested concentration causing 100% immobilisation within the 48 h test period was 0.50 mg/L.

The 48 h EC₅₀ of the test item was determined to be 0.262 mg/L (nominal).