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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005-11 to 2006-03
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions: analytical method no described in enough detail

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)
Version / remarks:
adopted May 1981
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
4,4-Difluoro Benzophenone
IUPAC Name:
4,4-Difluoro Benzophenone
Details on test material:
Physical state: white powder
Purity: 99%
Radiolabelling:
no

Sampling and analysis

Details on sampling:
- Sampling intervals/frequency for test organisms: at 30 h, 42 h, 72 h, 96 h, 120 h, 144 h, 240 h, 360 h
- Sampling intervals/frequency for test medium samples: at 0 h, 24 h, 48 h, 72 h, 96 h, 120 h, 144 h, 288 h
- Sample storage conditions before analysis: frozen
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):

Test solutions

Details on preparation of test solutions, spiked fish food or sediment:
A stock solution with a concentration of 100 mg/mL was prepared in acetone

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- fish were acclimated to laboratory conditons for 2 weeks
- length: 25 - 35 mm, weight: 0.2 - 0.4 g

Study design

Route of exposure:
aqueous
Test type:
semi-static
Water / sediment media type:
not specified
Remarks:
freshwater
Total exposure / uptake duration:
15 d
Total depuration duration:
8 d

Test conditions

Test temperature:
24 +/- 2 °C
pH:
6.95 to 7.04
Dissolved oxygen:
7.9 to 8.1 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- 120 L glass aquaria, filled with 100 L test solution
- Aeration: no:
- Renewal rate of test solution: every 24 hours
- No. of organisms per vessel: 150
- No. of vessels per concentration (replicates): 1
Nominal and measured concentrations:
Nominal: 0.1 mg/L and 0.2 mg/L
Details on estimation of bioconcentration:
- no data

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
55.4
Basis:
whole body w.w.
Time of plateau:
240 h
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0.1 mg/L
Type:
BCF
Value:
74.4
Basis:
whole body w.w.
Time of plateau:
240 h
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:0,2 mg/L
Depuration
Elimination:
yes

Any other information on results incl. tables

Concentration (mg/L)

Cf
(mg/kg, mean value)

Cw

(mg/L, mean value)

BCF

0.1

5.045

0.091

55.4

0.2

12.581

0.169

74.4

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

The bioaccumulation of 4,4-Difluoro Benzophenone was investigated in a bioaccumulation study with fish according to OECD 305 B. The test species Danio rerio was exposed to the test item at two concentration levels (0.1 and 0.2 mg/L) The uptake phase lasted 15 days (until the steady state was reached) and subsequent elimination was monitored over a period of 8 days. The following steady state BCF values were calculated

Conc. A (0.1 mg/L): BCF = 55.4

Conc. B (0.2 mg/L): BCF = 74.4

The study was assessed as "reliable with restrictions".